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H8666 Sigma-Aldrich

Transforming Growth Factor-β2 human

TGF-β2, recombinant, expressed in HEK 293 cells, HumanKine®, suitable for cell culture

Synonym: TGF-β2

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Reagents and Supplements, Recombinant TGF proteins,
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   0.1-0.5 ng/mL ED50
quality   endotoxin tested
mol wt   dimer mol wt 25 kDa (non-glycosylated)
packaging   pkg of 5 μg
mfr. no.   HumanZyme
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg
suitability   suitable for cell culture
UniProt accession no.   P61812
storage temp.   −20°C
Gene Information   human ... TGFB2(7042)

Description

Analysis Note

The specific activity was determined by the dose-dependent inhibition of IL-4 induced proliferation of mouse HT-2 (BALB/c spleen activated by sheep erythrocytes in the presence of IL-2).

Biochem/physiol Actions

Transforming growth factor-β2 (TGF-β2), like TGF-β1, is produced by many cell types and reported to be most concentrated in mammalian platelets.

TGF-β2 is important for immune homeostasis by balancing lymphocyte proliferation, apoptosis, hematopoiesis, and embryogenesis. TGF-β2 is crucial in cell growth, differentiation, and survival. TGF-β2 is a strong growth inhibitor for normal and transformed epithelial, lymphoid, fibroblast, and keratinocyte cells. TGF-β is associated with the process of tumor development, progression and metastasis. TGF-β2 is a tumor suppressor in the early stages of carcinogenesis, but in the later stages acts as a tumor promoter by inducing epithelial-mesenchymal transition and stimulating angiogenesis. TGF-β2 inhibits antitumor action of NK (natural killer) cells, T-cells, macrophages, monocytes and neutrophils. TGF-β is also associated with inflammation and wound healing. The TGF-β gene is overexpressed in glioma tumors and also in colon cancer, gastric cancer and cervical lesions.

Preparation Note

HumanKine TGF-β2 is expressed in human HEK 293 cells as a mature, non-glycosylated, disulfide-linked homodimer with a predicted molecular mass of approx. 25 kDa.

General description

Transforming growth factor-β2 (TGF-β2) belongs to the TGF-β superfamily. All threes isoforms (TGF-β1, TGF-β2 and TGF-β3) are known to share similar structure and function. The TGFB2 gene is mapped to human chromosome 1q41 and is localized to extracellular matrix. The active form of TGF-β precursor is produced upon proteolytic cleavage. TGF-β1 and TGF-β2 have 70% amino acid sequence identity.

Legal Information

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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