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  • H9384 - 22(R)-Hydroxycholesterol

H9384 Sigma



Synonym: 22α-Hydroxycholesterol, 5-Cholestene-3β,22(R)-diol, 5-Cholestene-3β,22[R]-diol



Related Categories Biochemicals and Reagents, Cell Biology, Cholesterol and Derivatives, Lipids, Metabolic Pathways,
assay   ≥98%



1, 5 mg in glass bottle

Preparation Note

22(R)-Hydroxycholesterol yield clear, colorless solution in chloroform.


Bovine aortic endothelial cells were treated with 22(R)-Hydroxycholesterol to study the effects on production of free radicals and in studies related to fatty acid metabolism.

Biochem/physiol Actions

22(R)-Hydroxycholesterol is an intermediate of the pregnenolone synthesis pathway from cholesterol. It reported has neuroprotective properties and protects the neurons against β-amyloid-induced cell death. 22(R)-Hydroxycholesterol acts as the ligand of liver X receptors that act as sensors of sterol concentration and regulates the fatty acid metabolism.

Other Notes

Tandem Mass Spectrometry data independently generated by Scripps Center for Metabolomics is available to view or download in PDF. H9384.pdf Tested metabolites are featured on Scripps Center for Metabolomics METLIN Metabolite Database. To learn more, visit sigma.com/metlin.

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Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

How does the storage temperature relate to shipping conditions?
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What is product H9384, 22(R)-Hydroxycholesterol, soluble in?  
Product H9384, 22(R)-Hydroxycholesterol, is reported to be soluble in chloroform (20mg/ml), absolute ethanol (20mg/ml), DMSO (0.1mg/ml) and dimethylformamide (2mg/ml).  Sigma-Aldrich tests solubility of this compound in chloroform at a concentration of 20mg/ml. 
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