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H9636 Sigma-Aldrich

Hypoxanthine

powder, BioReagent, suitable for cell culture

Synonym: 6-Hydroxypurine

  • CAS Number 68-94-0

  • Empirical Formula (Hill Notation) C5H4N4O

  • Molecular Weight 136.11

  •  Beilstein/REAXYS Number 5811

  •  EC Number 200-697-3

  •  MDL number MFCD00005725

  •  PubChem Substance ID 24895864

  •  NACRES NA.75

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Properties

Related Categories Cell Culture, Conditioned media supplements and reagents, Hybridoma Reagents, Miscellaneous Reagents and Supplements, Reagents and Supplements More...
Quality Level   200
product line   BioReagent
assay   ≥99%
form   powder
application(s)   cell culture | mammalian: suitable
mp   >300 °C (lit.)
solubility   formic acid: water (2:1): 50 mg/mL
SMILES string   O=C1NC=Nc2nc[nH]c12
InChI   1S/C5H4N4O/c10-5-3-4(7-1-6-3)8-2-9-5/h1-2H,(H2,6,7,8,9,10)
InChI key   FDGQSTZJBFJUBT-UHFFFAOYSA-N

Description

General description

Hypoxanthine (6-hydroxypurine), a purine derivative is a naturally occurring compound. It is the deaminated form of adenine and a breakdown product of adenosine monophosphate (AMP).

Application

Hypoxanthine is a nutrient additive for a variety of cell culture applications involving bacterial, parasite (Plasmodium falciparum) and animal cells. Hypoxanthine is a component of selection media used in hybridoma technologies.

Hypoxanthine has been used:
• as a supplement in HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) -buffered Roswell park memorial institute (RPMI) to culture Plasmodium falciparum strain IT/FCR3
• in the preparation of Dulbecco′s modified eagle′s medium (DMEM) + MXH for the selection of chimeric modified vaccinia virus ankara (MVA)
• to generate superoxide anions
• to prepare hybridoma selection and maintenance media.

Packaging

1, 5, 25 g in poly bottle

Biochem/physiol Actions

Hypoxanthine is capable of stimulating cell death. It can also induce reactive oxygen species (ROS). It results in endothelial dysfunction via apoptosis, stimulated by oxidative stress.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
UP0791000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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