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  • H9662 - Anti-hBRM/hSNF2α (LG-14) antibody produced in rabbit

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H9662 Sigma-Aldrich

Anti-hBRM/hSNF2α (LG-14) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Chromatin Remodeling, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 1:1,000 using extracts of 293T cells transfected with hBRM
species reactivity   human
mol wt   antigen 230 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
UniProt accession no.   P51531
Gene Information   human ... SMARCA2(6595)

Description

Immunogen

synthetic peptide corresponding to amino acids 1537-1550 of human BRM conjugated to KLH through an N-terminal added cysteine.

Application

Anti-hBRM/hSNF2a (KR-17) antibody produced in rabbit is suitable for:
• indirect immunofluorescence: 1μg/mL using paraformaldehyde/Triton fixed hBrm-transfected 293T cell line
• microarray
• western blot at a concentration of 0.15μg/mL using nuclear extracts of the 293T cell line

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Packaging

200 μL in glass insert

Biochem/physiol Actions

Chromatin remodeling complexes from various organisms, structurally contain related catalytic subunits, but differ in the way in which they manipulate chromatin. Three families of complexes have been described the SWI/SNF family, ISWI family, and Mi-2 family. The SWI/SNF family of ATP-dependent remodeling complexes was identified in yeast, drosophila, and human. It causes nucleosomes to change structure and/or position in order to allow transcriptional activators to gain access to their target sites. In humans, two conserved ATPase subunits have been identified as hBrm (also designated hSNF2α) and Brg1 (also designated as SNF2β). Components of the hSWI/SNF complexes have been implicated in a range of cellular events including gene activation, regulation of cell growth, and development. Brg1 and hBrm enhances transcriptional activation by glucocorticoid receptors. The remodeling complexes were traditionally associated with transcriptional activation. However, SWI/SNF has been found associated with repressor complexes, such as HDAC (histone deacetylase) and Rb (retinoblastoma) in a complex that leads to cell cycle arrest, suggesting that they are associated with transcriptional repression.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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