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H9664 Sigma

Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Gene Regulation, Antibodies in Chromatin Remodeling, Antibodies to Most Studied Epigenetic Genes,
species reactivity   mouse, human, rat
application(s)   immunoprecipitation (IP): 10 μg using HEK-293T cell lysates
  indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells.
  indirect immunofluorescence: suitable
  western blot: 1-2 μg/mL using 3T3 cell lysates
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 25 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)
biological source   rabbit
antibody product type   primary antibodies
conjugate   unconjugated

Description

Immunogen

synthetic peptide corresponding to amino acids 2-17 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

General description

HMGB proteins belong to the High Mobility Group (HMG) family of proteins that contain the HMG-box for binding and changing DNA structures . HMGB1 has been implicated in cellular processes such as DNA recombination and repair, and also in mRNA synthesis . Increased expression of HMGB1 is observed in cancer cells . Anti-HMGB1 (HMG1) (N-terminal) antibody is specific for human, mouse and rat HMGB1. Detection of the 25 kDa HMGB1 band by immunoblotting is specifically inhibited with the immunizing peptide.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Application

Anti-HMGB1 (HMG1) (N-terminal) antibody is suitable for use in chemiluminescent immunoblot (using mouse heart homogenates) . The antibody can also be used in immunoprecipitation (10 μg using HEK-293T cell lysates), indirect immunofluorescence (1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells), and western blot (1-2 μg/mL using 3T3 cell lysates).

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about the Sigma Life Science and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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