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H9666 Sigma-Aldrich

Nerve Growth Factor β Human

NGF-β, recombinant, expressed in HEK 293 cells, HumanKine, suitable for cell culture

Synonym: NGF-β human

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, HumanKine Growth Factors and Cytokines, Neurotrophic Factors, Neurotrophin family - includes nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrohin-3 (NT-3) and neurotrohin-4 (NT-4),
biological source   human
recombinant   expressed in HEK 293 cells
assay   ≥95% (SDS-PAGE)
form   lyophilized powder
potency   ≤16 ng/mL ED50
quality   endotoxin tested
mol wt   13 kDa (non-glycosylated)
packaging   pkg of 10 μg
mfr. no.   HumanZyme
impurities   ≤1 EU/μg
suitability   suitable for cell culture

Description

General description

HumanKine NGF-β is expressed in human 293 cells as a non-glycosylated, non-disulfide bonded 13 kDa homodimer.

NGF-β belongs to the nerve growth factor protein superfamily. It is a key growth factor in target-mediated regulation of peripheral innervation. During development, NGF-β regulates nerve cell death, synaptic connectivity, fiber guidance, and dendritic morphology. The presence of NGF-β in immune cells and endocrine cells as well as in the CNS limbic areas suggests that it may function as an intracellular messenger to regulate the body′s response to stress.

The nerve growth factor β (NGFB) gene is mapped to human chromosome 1p13.2.

Application

Nerve Growth Factor β Human has been used as a neurotrophic agent in luciferase reporter assay using mammalian cells.

Biochem/physiol Actions

Nerve Growth Factor β (NGFB) acts as a key mediator in neuronal and non-neuronal development, differentiation, and survival. Polymorphism of NGFB gene leads to hereditary sensory and autonomic neuropathies. Charcot arthropathy and thermal insensitivity are few of the related symptoms. NGFB is known to promote cancer cell proliferation and metastasis. It is associated with lung, breast, prostatic and pancreatic cancers. In animal models, NGFB is observed to prevent cholinergic neuron loss and enhances memory.

Analysis Note

The specific activity was determined by the dose-dependent stimulation of the proliferation of the human TF-1 cells (human erythroleukemic indicator cell line).

Legal Information

3D Insert is a trademark of 3D Biotek, LLC

HumanKine is a registered trademark of HumanZyme Inc.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

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Peer-Reviewed Papers
15

References

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