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HPA003336 Sigma

Anti-DUSP9 antibody produced in rabbit Antibody Enhanced Validation

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym: Anti-Mitogen-activated protein kinase phosphatase 4 antibody produced in rabbit, Anti-Dual specificity protein phosphatase 9 antibody produced in rabbit, Anti-MAP kinase phosphatase 4 antibody produced in rabbit, Anti-MKP-4 antibody produced in rabbit



Related Categories Alphabetical Index, Antibodies, D, DQ-DZ, Prestige Antibodies,
species reactivity   human
application(s)   immunohistochemistry: 1:50- 1:200
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous glycerol solution
grade   Prestige Antibodies® Powered by Atlas Antibodies
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... DUSP9(1852)
biological source   rabbit
antibody product type   primary antibodies
enhanced validation   orthogonal RNAseq
Learn more about Antibody Enhanced Validation
conjugate   unconjugated



Dual specificity protein phosphatase 9 recombinant protein epitope signature tag (PrEST)

General description

DUSP9 (dual specificity phosphatase 9) is predominantly expressed in the placenta and developing liver during early development and organogenesis. DUSP9 (dual specificity phosphatase 9) is predominantly expressed in the placenta and developing liver during early development and organogenesis.

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide


All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.

Legal Information

Prestige Antibodies is a registered trademark of Sigma-Aldrich Co. LLC

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


Corresponding Antigen APREST74382.

Biochem/physiol Actions

DUSP9 (dual specificity phosphatase 9) gene encodes a cytoplasmic protein belonging to the dual specificity protein phosphatase subfamily. These proteins dephosphorylate both the phosphoserine/threonine and phosphotyrosine residues in the target kinases and deactivate them. Dusp9 preferentially recognizes and inactivates classical ERK1 (extracellular signal-regulated kinase-1) and -2 MAPKs (mitogen-activated protein kinases) in mammalian cells that are associated with cellular proliferation and differentiation. It protects against the development of insulin resistance by inactivating the mediators involved in stress-induced insulin resistance. It may serve as a therapeutic target for insulin-resistant disorders.

Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, half-mask respirator (US), multi-purpose combination respirator cartridge (US)
RIDADR  NONH for all modes of transport
WGK Germany  1
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Prestige Antibody® Immunofluorescence Procedure

Prestige Antibodies® have been used for subcellular localization studies by immunocytochemistry (ICC) - immunofluorescence (IF) using the protocol described below. In addition to the primary antibodi...
Keywords: Immunocytochemistry, Immunofluorescence, Immunostaining, Sample preparations

Prestige Antibody® Immunohistochemistry Procedure

Prestige Antibodies® developed for immunohistochemistry based expression profiling are recommended to be used according to the standard IHC staining protocol described below.
Keywords: Buffers, Dehydration reaction, Gene expression, Hydration reaction, Immunohistochemistry, Immunostaining, Tissue microarrays

Prestige Antibody® Protein Immunoblotting Procedure

Prestige Antibodies® recommended for Western Blot are recommended to be used according to the standard WB protocol described below. Western blot standard protocol optimized for
Keywords: Catalytic combustion detector, Electrophoresis, Gene expression, Sample preparations, Western blot

Prestige Antigens™-Blocking Protocol

Prestige Antigens and the corresponding Prestige Antibodies are recommended to be used according to the standard blocking protocol described below.
Keywords: Immunocytochemistry, Immunohistochemistry, Immunostaining, Titrations, Western blot

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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免疫球蛋白(Ig)由B淋巴细胞产生并分泌到血浆中。单体形式的Ig分子是一个分子量约为150 kDa的糖蛋白,且其结构差不多类似字母Y。Ig单体的基本结构(图1)包括由两个二硫键连接的两个相同的半体。每个半体都具有一个约50 kDa的重链和一个约25 kDa的轻链,两者在轻链羧基末端附近通过二硫键连接在一起。重链可分为Fc部分和Fab部分,前者位于羧基末端(Y的基座),后者则位于氨基末端(Y的臂)...
Keywords: Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Rheumatology

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