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HPR116080 Sigma-Aldrich

Differentiated HepaRG Cells Cryopreserved

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Properties

Related Categories ADME/Tox Products, Cell Biology, Cell Line Models, Liver Drug Metabolism Knockout Models, Liver Drug Transporter/Toxicity Models More...
biological source   (Liver tumor of female patient diagnosed with hepatocarcinoma and hepatitis C)
shipped in   dry ice
storage temp.   −196°C

Description

General description

HepaRG is a human hepatoma cell line originally derived from a female patient diagnosed with hepatocarcinoma and hepatitis C. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line that has the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG cells are useful as an alternative to human primary cells as they retain the major functional activities of these cells. HepaRG cells express functional levels of the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways. HepaRG cells are fully differentiated and ready to use upon receipt.

Application

See user guide for detailed protocols.

Features and Benefits

HepaRG cells are useful for many in vitro ADME applications, including drug metabolism,reactive metabolite formation, clearance, CYP inhibition and induction assays. They are also suitable for a variety of in vitro toxicity assays, including general hepatotoxicity, cholestasis,and viral infection. HepaRG cells have recently been grown in bioreactors, used for the generation of hepatic spheroids and been implanted into animals to produce humanized livers. The potential range of applications for HepaRG cells continues to expand at a rapid pace.

Legal Information

Use of this product is subject to one or more license agreements.

HepaRG cells are patented and their use is strictly limited; consider the cells as a single use, disposable product that must be destroyed upon conclusion of a study or experiment. Propagating, reproducing, cloning, subcloning or any other use of the cells following the conclusion of a study is prohibited. Use of the cells to produce or manufacture commercial products for general sale or for use in the manufacture of products intended for general sale is prohibited. Transfer of the cells to anyone not employed within the same organization, whether for financial benefit or not, is prohibited. If you are unwilling to accept the terms of this LIMITED USE LICENSE, do not ORDER or use them, and immediately return the cells for credit. Violators of this Limited Use License will be prosecuted to the fullest extent of the law.

HepaRG is a trademark of BioPredic International company

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

The Role of Liver Transporters in Drug-Drug Interactions

Oral drug delivery involves dissolution in the small intestine and absorption across the enterocyte barrier into the portal vein followed by subsequent delivery through the liver into the systemic ci...
David C. Thompson, Ph.D, R&D Manager, Sigma® Life Science and Michael D. Mitchell, Product Manager, Sigma® Life Science
Biofiles Vol. 8, No. 12
Keywords: Absorption, Cancer, Clinical, Eliminations, Genetic, Metabolic Pathways, Metabolism, Pharmaceutical, Polymorphisms

Peer-Reviewed Papers
15

References

Related Products

supplement

Product #

Description

Add to Cart

ADD670C HepaRG Thawing and Plating Meduim Supplement with Antibiotics
ADD620C HepaRG Maintenance and Metabolism Medium Supplement with Antibiotics
ADD640C HepaRG Induction Medium Supplement with Antibiotics
ADD650C HepaRG Serum Free Induction Medium Supplement with Antibiotics
ADD671C HepaRG Thawing and Plating Medium Supplement
ADD621C HepaRG Maintenance and Metabolism Medium Supplement
ADD641C HepaRG Induction Medium Supplement
ADD651C HepaRG Serum Free Induction Medium Supplement

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