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  • HSANGERG - Sanger Arrayed Whole Genome Lentiviral CRISPR Library



Related Categories CRISPR-Cas9, Functional Genomics and RNAi, Molecular Biology, Sanger Libraries
Quality Level   200
packaging   pkg of 50 μL (96-well plate)
application(s)   CRISPR: suitable
shipped in   dry ice
storage temp.   −70°C


General description

Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.


Functional Genomics/Screening /Target Validation

Features and Benefits

• Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
• Simplify the workflow with puromycin selection
• Illuminate CRISPR-expressing cells with BFP
• Flip the expression components into and out of the genome using transposase

Additional Features
• Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
• Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
• Collaborative: Real-time, library validation continues

For detailed information on the Sanger library, click here


96-well plates


Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones

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Physical form

50 μl of bacterial glycerol stocks, 383×96-well plates

Other Notes

This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling.

Recommended products

Additional libraries are available:
Mouse, Glycerol Format
Human, Virus Format
Mouse, Virus Format

Legal Information

CRISPR Use License Agreement

Lentiviral License Agreement

Safety & Documentation

Safety Information

UN 3245 9
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis

Protocols & Articles


Sanger Sequencing Steps | DNA Sequencing

Sanger sequencing, also known as the “chain termination method”, is a method for determining the nucleotide sequence of DNA. The method was developed by two time Nobel Laureate Frederick Sanger and h...
Keywords: Electrophoresis, Polymerase chain reaction, Sequencing

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In recent years CRISPR has revolutionized gene editing capabilities, leading to sophisticated ways to create success with any experiment. As the first company to offer custom biomolecules globally fo...
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Predictive Models for Neuroscience using CRISPR [VIDEO]

Caroline Beckett, the global CRISPR product manager, discusses reagent solutions for creating predictive models for neuroscience research. She notes that many neuroscientists want to be able to creat...
Keywords: Cell culture, Diseases, Neurodegenerative Diseases, Neuroscience

Peer-Reviewed Papers


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