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HT501128 Sigma-Aldrich

Formalin solution, neutral buffered, 10%

histological tissue fixative

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Properties

Related Categories Cell Biology, Core Bioreagents, Fixatives, Hematology and Histology, Hematology and Histology Lab Accessories,
InChI Key   WSFSSNUMVMOOMR-UHFFFAOYSA-N
shelf life   Expiry date on the label.
concentration   10% formalin (approx. 4% formaldehyde)

Description

Application

Popular general purpose histological fixative.

Formalin solution, neutral buffered, 10% has been used as a popular general purpose histological fixative to fix tissues.

Formalin solution, neutral buffered, 10% has been used in oil-red-o staining for the differentiation of 3T3-L1 preadipocyte cell line and mouse embryonic fibroblasts into adipocytes. It has been used in the histological analysis of rabbit′s eye and diabetic rat intestine.

Physical form

Neutral buffered solution

General description

10% Neutral buffered formalin (NBF) is the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. 10% NBF is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue. Formalin fixation is thought to form between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
WGK Germany 
3
Flash Point(F) 
185 °F
Flash Point(C) 
85 °C

Documents

Certificate of Analysis

Certificate of Origin

Protocols & Articles

Articles

Bile Acids

The liver excretes excess cholesterol in the form of bile acids. Bile acids serve two purposes: to remove unwanted cholesterol from the body and to aid in lipid digestion in the intestine. Bile acids...
BioFiles 2007, 2.7, 17.
Keywords: Absorption, Digestions, Transcription

Peer-Reviewed Papers
15

References

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