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HT5011 Sigma-Aldrich

Formalin solution, neutral buffered, 10%

case of 48 × 15 mL, histological tissue fixative

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Properties

Related Categories Cell Biology, Fixatives, Hematology and Histology, Tissue Processing
shelf life   Expiry date on the label.
packaging   case of 48 × 15 mL
concentration   10% formalin (approx. 4% formaldehyde)
application(s)   hematology: suitable
  histology: suitable
color   colorless
pH   6.90-7.10
density   1.080 g/cm3
storage temp.   room temp
InChI   1S/CH2O/c1-2/h1H2
InChI key   WSFSSNUMVMOOMR-UHFFFAOYSA-N

Description

General description

Formaldehyde is both a non-coagulant and an additive fixative. Formaldehyde is known to penetrate tissue quickly, but the fixation process may take a long time to form the various cross links with tissue proteins. Formalin fixation is thought to form cross links between the aldehydes and the proteins, creating a gel, thus retaining cellular constituents in their in vivo relationship. Once properly fixed, the tissue should be able to withstand the subsequent stages of tissue processing or staining. Although there is no true universal fixative, 10% neutral buffered formalin (NBF) is perhaps the most commonly used fixative throughout the world for light microscopy and is a somewhat forgiving fixative. Of the dozens and dozens of available fixatives, it is hard to imagine a fixative more commonly used than 10% NBF. 10% neutral buffered formalin is well suited for large throughput laboratories, and requires a relatively short period of fixation, but can also be used for the long-term storage of tissue.

Application

10% neutral buffered formalin (NBF) is a popular general purpose histological fixative. It has been used to fix tissues, protein complexes and tumor samples.

Popular general purpose histological fixative.

Packaging

Case containers are polypropylene with white leak-proof lids and are partially prefilled with 10% NBF.

Physical form

Neutral buffered solution

Storage and Stability

Store tightly closed at room temperature. Do not freeze.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
WGK Germany 
WGK 3
Flash Point(F) 
185.0 °F
Flash Point(C) 
85 °C

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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HT501128 Formalin solution, neutral buffered, 10%, histological tissue fixative

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