• USA Home
  • HT901 - Crystal violet solution

HT901 Sigma-Aldrich

Crystal violet solution

  • CAS Number 548-62-9

  • Empirical Formula (Hill Notation) C25H30ClN3

  • Molecular Weight 407.98

  •  Beilstein/REAXYS Number 3580948

  •  MDL number MFCD00011750

  •  PubChem Substance ID 24895895

  •  NACRES NA.47



Related Categories Cell Biology, Hematology and Histology, Histology Special Stains More...
Quality Level   500
form   solution
shelf life   Expiry date on the label
IVD   for in vitro diagnostic use
concentration   2.3%
application(s)   hematology: suitable
  histology: suitable
pH   4.6
storage temp.   room temp
SMILES string   [Cl-].CN(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](/C)C
InChI   1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1



Kit component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).


HT901 is composed of 2.3% certified crystal violet, 0.1% ammonium oxalate monohydrate, and 20% ethyl alcohol, SD3A.

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
UN 1993C 3 / PGIII
WGK Germany 


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
Is Product HT901, Crystal violet solution, used as part of a kit?
Although available separately, this solution is also a component of Gram Stain kits HT90A and HT90T (for staining of heat-fixed films and tissue, respectively).
Is Product HT901, Crystal violet solution, suitable for colony staining, migration or plaque staining?
We recommend Product No. C0775 (Crystal Violet, certified by the Biological Stain Commission), or Product No. 93770 (Tuerk's solution) for these stains, rather than diluting down the relatively high concentration of Crystal Violet (2.3%) in HT901 to approximately 0.1%. Product HT901 was formulated for use in the Gram Stain procedure. The product's performance has not been tested for use in colony staining, migration or plaque assays.
What is the formulation of Product HT901, Crystal violet solution?
HT901 is composed of:certified Crystal Violet, 2.3%Ammonium Oxalate, 0.1%20% denatured ethyl alcohol.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
Show more questions
Protocols & Articles
Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?