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HYS020 Sigma-Aldrich

HyStem® Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution




HyStem® cell culture scaffold kit has been successfully used to expand human embryonic stem cells (H9s), umbilical cord blood CD23+ stem cells, hepatic stem cells and hepatic progenitor cells.

Features and Benefits

Customizable and free of animal components, Hystem hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
• Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
• Animal Component Free - As a synthetic Hyaluronic acid-based matrix, Hystem offers complete control of the cellular environment. All components are well-characterized, consistent, and derived from non-animal sources
• Biologically accurate - Rich in hyaluronic acid, the Hystem hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

With Hystem, researchers can tailor the incorporation of ECM proteins, cell attachment factors, and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem is recommended for both in vivo and in vitro experimentation.

Hystem offers a minimal number of cell attachment sites. If desired, researchers may incorporate attachment factors of their choice.

Legal Information

Hystem is a registered trademark of BIOTIME, INC

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis


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Milli-Q® Water Purification Solutions
Protocols & Articles


3d Cell Culture: Tools and Techniques

Cell culture techniques are ubiquitous in areas of developmental biology, drug discovery, regenerative medicine and protein production. Since the introduction of cell culture techniques, cells have b...
Keywords: Alkylations, Apoptosis, Biomaterials, Cancer, Cell culture, Cell proliferation, Clinical, Cytotoxins, Drug discovery, Gene expression, Metabolism, Pharmaceutical

Cell Culture FAQs: Bacterial Endotoxin Contamination

Endotoxins are small bacterially-derived hydrophobic lipopolysaccharide (LPS) molecules that can easily contaminate labware and whose presence can significantly impact both in vitro and in vivo exper...
Keywords: Apoptosis, Cell culture, Cell disruption, Filtration, Growth factors, Hormones, Purification, Pyrogens, Sterilizations

Heparin Vs Heparin Sulfate Proteoglycans

Heparan sulfate (HS) is a component of the extracellular matrix. It is a glycosaminoglycan that is covalently attached to core proteins to form proteoglycans. Its structure is amazingly diverse since...
Keywords: Adhesion, Anticoagulants, Epimerizations, Inflammation, Ligands, Sulfations

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Introduction HyStem™ Features and Benefits Protocols and Support Publications using HyStem™ with Applications Examples of Cells That Have Been Cultivated Successfully with HyStem™ Platform
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cell proliferation, Chromatin immunoprecipitation, Growth factors, Hydration reaction, Neuroscience

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

What is Hyaluronan?

Hyaluronic acid (HA) is the simplest glycosaminoglycan (a class of negatively charged polysaccharides) and a major constituent of the extracellular matrix (ECM).1 Hyaluronan is a scaffold secreted by...
Keywords: Adhesion, Angiogenesis, Cancer, Cell culture, Cell proliferation, Cloning, Degradations, Deposition, Digestions, Eliminations, Fermentation, Gene expression, Glycosylations, Growth factors, Hydration reaction, Metabolism, Palmitoylations, Phosphorylations, Purification, Sonication, Transcription, Transfection

What is a Cell Delivery Vehicle?

A cell delivery vehicle is a matrix (made from natural or synthetic materials or a combination of the two) that can be combined with cells to be transplanted into a human or animal host.
Keywords: Apoptosis, Cell attachment, Clinical, Degradations, Growth factors, Transduction

Peer-Reviewed Papers


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HYS010 HyStem® Cell Culture Scaffold Kit, Trial Kit, For 2.5 mL of hydrogel scaffold solution
92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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