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HYSHP020 Sigma-Aldrich

HyStem®-HP Cell Culture Scaffold Kit

For 7.5 mL of hydrogel scaffold solution



General description

The HyStem®-HP Cell Culture Scaffold Kit provides an excellent starting point for optimizing the matrix for stem cell culture, where slowly released growth factors are crucial in re-creating a stem cell niche. To affect specific cell performance, growth factors or ECM proteins may be added to the HyStem-HP hydrogel. Unlike animal-derived ECM products, this kit contains three fully chemically defined components, which are nonimmunogenic:
• HyStem®-HP – a thiol-modified hyaluronan (a major constituent of native ECM), carboxymethyl hyaluronic acid-thiopropanoyl hydrazide (CMHA-S, CMHA-DTPH, carboxymethyl hyaluronic acid-DTPH) with heparinthiopropanoyl hydrazide (HP-DTPH). HyStem-HP (CMHA-DTPH with HP-DTPH) contains 99.7 wt% CMHA-DTPH and 0.3 wt% HP-DTPH.
• Gelin-S – a thiol-modified gelatin (denatured collagen), carboxymethyl gelatin-thiopropanoyl hydrazide (GTN-DTPH, carboxymethyl gelatin-DTPH)
• Extralink® – a thiol-reactive crosslinker, polyethylene glycol diacrylate (MW = 3,400 g/mole, PEGDA)

Features and Benefits

Customizable, well characterized and consistent, Hystem-HP hydrogel cell culture scaffolds provide a complex, three dimensional environment in which cells are able to proliferate, much as they would in vivo.
• Customizable - each distinct cell type in a multicellular organism has its own unique microenvironment. Hystem-HP offers the flexibility to tailor the environments of cultured cells to mimic their in vivo counterparts and optimize proliferation and differentiation.
• Synthetic - As a synthetic matrix, Hystem-HP offers greater control of the cellular environment than ambiguous extracted ECM alternatives. All components are well characterized and consistently formulated. Hystem-HP contains Hyaluronic acid (synthetic), Extralink (synthetic), Gelin-S (porcine), Heparin and Water.
• Biologically accurate - Rich in hyaluronic acid and denatured collagen, the Hystem-HP hydrogel closely mimics the complex three dimensional cellular environments found in life, providing an optimal environment for culturing your cells.

Hystem-HP enables ionic binding and slow release of growth factors through thiolated heparin; growth factors can be integrated via the matrix rather than cell culture medium. Hystem-HP also enables the attachment of a wide variety of cell types through Gelin-S (thiolated gelatin).

With Hystem-HP, researchers can tailor the incorporation of ECM proteins and growth factors as well as the elasticity of the hydrogel and cell incorporation (encapsulations or top plating) to create the specific 3D environment required by their cells.

Hystem-HP is recommended for both in vivo and in vitro experimentation.

Legal Information

Extralink is a registered trademark of Glycosan BioSystems, Inc.

Gelin is a trademark of Biopharm Ltd.

Hystem is a registered trademark of BIOTIME, INC

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport


Certificate of Analysis


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Milli-Q® Water Purification Solutions
Protocols & Articles


3d Cell Culture: Tools and Techniques

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Cell Culture FAQs: Bacterial Endotoxin Contamination

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Heparin Vs Heparin Sulfate Proteoglycans

Heparan sulfate (HS) is a component of the extracellular matrix. It is a glycosaminoglycan that is covalently attached to core proteins to form proteoglycans. Its structure is amazingly diverse since...
Keywords: Adhesion, Anticoagulants, Epimerizations, Inflammation, Ligands, Sulfations

HyStem™ Hyaluronic Acid Based Hydrogels for 3D Cell Culture Applications

Introduction HyStem™ Features and Benefits Protocols and Support Publications using HyStem™ with Applications Examples of Cells That Have Been Cultivated Successfully with HyStem™ Platform
Keywords: Adhesion, Cancer, Cell attachment, Cell culture, Cell proliferation, Chromatin immunoprecipitation, Growth factors, Hydration reaction, Neuroscience

Hydrogels for 3D cell culture

Cells in their natural environment are surrounded by a complex network of extracellular molecules called Extracellular Matrix (ECM). This network provides structure and function, in the form of bioch...
Keywords: Adhesion, Cancer, Cell culture, Growth factors

What is Hyaluronan?

Hyaluronic acid (HA) is the simplest glycosaminoglycan (a class of negatively charged polysaccharides) and a major constituent of the extracellular matrix (ECM).1 Hyaluronan is a scaffold secreted by...
Keywords: Adhesion, Angiogenesis, Cancer, Cell culture, Cell proliferation, Cloning, Degradations, Deposition, Digestions, Eliminations, Fermentation, Gene expression, Glycosylations, Growth factors, Hydration reaction, Metabolism, Palmitoylations, Phosphorylations, Purification, Sonication, Transcription, Transfection

What is a Cell Delivery Vehicle?

A cell delivery vehicle is a matrix (made from natural or synthetic materials or a combination of the two) that can be combined with cells to be transplanted into a human or animal host.
Keywords: Apoptosis, Cell attachment, Clinical, Degradations, Growth factors, Transduction

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HYSHP010 HyStem®-HP Cell Culture Scaffold Kit, Trial Kit, For 2.5 mLof hydrogel scaffold solution

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