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  • I0282 - Anti-α-Internexin antibody, Mouse monoclonal

I0282 Sigma-Aldrich

Anti-α-Internexin antibody, Mouse monoclonal

clone 2E3, purified from hybridoma cell culture

Synonym: Anti-66 kDa Neurofilament Subunit, Anti-INA, Anti-NF-66



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Neural Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   2E3, monoclonal
biological source   mouse
application(s)   immunocytochemistry: suitable
  immunohistochemistry: suitable
  indirect ELISA: suitable
  microarray: suitable
  western blot: 0.5 μg/mL using rat brain cytosolic extract
species reactivity   rat, bovine, human, feline, pig
mol wt   antigen ~66 kDa by SDS-PAGE
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified from hybridoma cell culture
isotype   IgG1
antibody product type   primary antibodies
concentration   ~2 mg/mL
UniProt accession no.   Q16352
Gene Information   human ... INA(9118)
rat ... Ina(24503)


General description

Monoclonal Anti-α -Internexin (mouse IgG1 isotype) is derived from the 2E3 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mice immunized with the full-length recombinant rat α -internexin.

The gene INA (α-Internexin) encodes a neuronal intermediate filament (IF) protein that is a type IV IF. The gene is mapped to human chromosome 10. The encoded protein has a molar mass of 55.4kDa and spans a length of 499 amino acids. It is expressed in the neurons along with the NF (neurofilament) triplet proteins, NF-L (light), NF-M (medium), NF-H (heavy).


full-length recombinant rat α-internexin.


Anti-α-Internexin antibody, Mouse monoclonal has been used in:
• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunohistochemistry
• immunocytochemistry

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The expression of α-Internexin precedes the expression of NF triplet proteins during the differentiation of neurons. Its expression is relatively low when compared to the the NF proteins in the adult brain. It co-assembles with the NF triplet proteins. Overexpression of α-internexin in transgenic mouse model has been found to cause abnormal neurofilamentous accumulations and motor coordination deficits. It has been found to be present in cytoplasmic inclusions linking it to NIFID (neuronal intermediate filament inclusion disease) and other neurological diseases with pathological accumulations of IFs (intermediate filaments). α-Internexin may be useful as a biomarker for pancreatic neuroendocrine tumor aggressiveness and prognosis.

Safety & Documentation

Safety Information

WGK Germany 
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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