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I0406 Sigma-Aldrich

Interleukin-6 from rat

IL-6, recombinant, expressed in E. coli, buffered aqueous solution, cell culture tested

Synonym: Interleukin-6 from rat

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Description

Biochem/physiol Actions

Interleukin-6 (IL-6) is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Physical form

Supplied as a 0.2 μm filtered solution in 50 mM sodium acetate, pH 4, with BSA as a carrier protein.

Preparation Note

Recombinant, rat Interleukin-6 is produced from a DNA sequence encoding the mature rat IL-6 protein. The methionyl form of mature rat IL-6, a 188 amino acid protein, has a predicted molecular mass of approx. 22 kDa. Rat and mouse IL-6 share 93% amino acid sequence identity.

Analysis Note

The biological activity is measured in a cell proliferation assay using IL-6 dependent mouse plasmacytoma cell line, T1165.85.2.1.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

Articles

Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages...
Jennifer Fries
BioFiles 2009, 4.5, 16.
Keywords: Anti-inflammatory agents, Antitumor agents, Cancer, Cell proliferation, Gene expression, Growth factors, Transcription

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Peer-Reviewed Papers
15

References

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SRP4145 IL-6 from rat, recombinant, expressed in E. coli, ≥90% (SDS-PAGE), ≥90% (HPLC)
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