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I0516 Sigma-Aldrich

Insulin solution from bovine pancreas

BioReagent, 10 mg/mL insulin in 25  mM HEPES, pH 8.2, sterile-filtered, suitable for cell culture

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Properties

Related Categories Cell Culture, Hormones, Reagents and Supplements More...
Quality Level   500
sterility   sterile-filtered
product line   BioReagent
form   solution
concentration   10 mg/mL insulin in 25  mM HEPES, pH 8.2
application(s)   cell culture | mammalian: suitable
impurities   endotoxin, tested
storage temp.   2-8°C

Description

Application

Recommended for use in cell culture applications at 0.5 to 1 mL per liter of medium.

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Physical form

stable 2-8 °C

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
What is the comparison between International Units (IU) and USP units for insulin?
The international unit (IU) of insulin is based on the International Standard Preparation of Bioassay (1958). The standard preparation is a mixture of 52% bovine and 48% porcine insulin, containing 24 units/mg. Reference: Martindale: The Extra Pharmacopoeia, 29th ed., p. 392. High purity bovine pancreatic insulin typically assays at approximately 27 USP units per mg. So the IU and USP unit are not identical, but they are approximately 1:1.
What is the amino acid sequence for insulin?
The sequence and structure of insulin can be found on the product information sheet (under Documents, above).
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How many units/mg am I getting in Product I0516, Insulin solution?
This solution is made using insulin Product No. I5500.The potency specification for the I5500 is not less than 27 USP units/mg.However, we do not test for potency of the final product of this solution.It is possible some activity is lost during the manufacturing process.
What is the molarity of Product I0516, Insulin solution?
Product No. I0516 is a 10 mg/mL solution of bovine insulin (MW 5733.5). The molarity is 1.7 μmol/mL or 1.7 mM.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
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References

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