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I1149 Sigma

Iodoacetamide

BioUltra

  • CAS Number 144-48-9

  • Linear Formula ICH2CONH2

  • Molecular Weight 184.96

  •  Beilstein Registry Number 1739080

  •  EC Number 205-630-1

  •  MDL number MFCD00008028

  •  PubChem Substance ID 24278486

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Properties

Related Categories A to C, Biochemicals and Reagents, Carnitine acetyltransferase, Core Bioreagents, D to K,
InChI Key   PGLTVOMIXTUURA-UHFFFAOYSA-N
Quality Level   PREMIUM
product line   BioUltra
assay   ≥99% (NMR)
impurities   ≤0.0005% Phosphorus (P)
  ≤0.1% Insoluble matter
mp   92-95 °C(lit.)
solubility   H2O: 0.5 M, clear, colorless
anion traces   chloride (Cl-): ≤0.05%
  sulfate (SO42-): ≤0.05%
cation traces   Al: ≤0.0005%
  Ca: ≤0.0005%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
storage temp.   2-8°C

Description

Application

Alkylating reagent for cysteine residues in peptide sequencing. By virtue of reaction with cysteine, it is an irreversible inhibitor of enzymes with cysteine at the active site. It reacts much more slowly with histidine residues, but that activity is responsible for inhibition of ribonuclease.

Packaging

5, 25, 100 g in glass bottle

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
UN 2811 6.1 / PGIII
WGK Germany 
3
RTECS 
AC4200000

Expore Enzymes, Proteins & Cofactors

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Protocols & Articles

Protocols

In-gel digestion for mass spectrometric characterization of proteins and proteomes

In-gel digestion of proteins isolated by gel electrophoresis is a cornerstone of mass spectrometry (MS)-driven proteomics. The 11-year-old recipe by Shevchenko et al. has been optimized to increase t...
Keywords: 2D Electrophoresis, Alkylations, Biochemistry, Cell biology, Cell culture, Cell disruption, Chromatography, Condensations, Dehydration reaction, Detergents, Diffusion, Digestions, Electrophoresis, Gel electrophoresis, Gene expression, Genetics, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Phase transitions, Polymerase chain reaction, Proteomics, Reductions, Separation, Sequencing, Sonication

State-of-the-art two-dimensional gel electrophoresis: a key tool of proteomics research

Odile Carrette a , Pierre R Burkhard a, b , Jean-Charles Sanchez a , Denis F Hochstrasser a, c, d
Keywords: 2D Electrophoresis, Absorption, Alkylations, Carcinogens, Clinical, Clinical Chemistry, Deposition, Detergents, Electrophoresis, Gel electrophoresis, High performance liquid chromatography, Immobilization, Isoelectric focusing, Mutagens, Neurology, PAGE, Polymerization reactions, Precipitation, Protein assay, Protein electrophoresis, Proteomics, Reductions, Respiratory, Sample preparations, Separation, Size-exclusion chromatography, Teratogens

Peer-Reviewed Papers
15

References

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