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  • I157 - Anti-Inositol 1,4,5-Triphosphate Receptor (Type I) antibody produced in rabbit

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I157 Sigma-Aldrich

Anti-Inositol 1,4,5-Triphosphate Receptor (Type I) antibody produced in rabbit

whole antiserum, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Calcium Channels, Antibodies to Ion Channels,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunohistochemistry (frozen sections): 1:100
  immunoprecipitation (IP): suitable
  western blot: 1:1,000
species reactivity   mouse, human, rat
mol wt   antigen ~240 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   whole antiserum
antibody product type   primary antibodies
UniProt accession no.   Q14643
Gene Information   human ... ITPR1(3708)
mouse ... Itpr1(16438)
rat ... Itpr1(25262)

Description

General description

The gene ITPR1 (inositol 1,4,5-triphosphate receptor type 1) encodes an intracellular receptor that binds to inositol 1,4,5 trisphosphate. It is localized to membranes of endoplasmatic or sarcoplasmatic (in muscle cells) reticulum. The gene is mapped to human chromosome 3p26.

Specificity

The antibody reacts specifically with IP3R-I in microsomes prepared from liver epithelial cells, cerebellum, and vascular smooth muscle cells.

Immunogen

synthetic peptide derived from amino acids 1829-1848 of cytoplasmic C-terminal domain of human inositol 1,4,5-triphosphate receptor, type I (IP3R-I). This sequence is 100% conserved in human, mouse and rat IP3R-I.

Physical form

Solution in phosphate buffered saline containing 0.05% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The ligand-gated calcium channel encoded by the gene ITPR1 (inositol 1,4,5-triphosphate receptor type 1) is stimulated upon binding to its ligand inositol 1,4,5-triphosphate, and mediates intracellular calcium signaling. It facilitates the release of Ca2+ from endoplasmic reticulum and modulates intracellular Ca2+ concentration. This is a crucial mechanism in the initial stages of myoblast differentiation. Mutations in this gene have been associated with sporadic infantile-onset cerebellar ataxia.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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