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  • I1654 - Anti-IRAK2 (546-564) antibody produced in rabbit

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I1654 Sigma-Aldrich

Anti-IRAK2 (546-564) antibody produced in rabbit

~0.5 mg/mL, affinity isolated antibody

Synonym: Anti-Interleukin-1 Receptor-Associated Kinase 2

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Epigenetics and Nuclear Signaling, Antibodies for Gene Regulation,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL using HeLa or K562 cell lysates
species reactivity   human
mol wt   antigen mol wt 65 kDa
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
concentration   ~0.5 mg/mL
UniProt accession no.   O43187
Gene Information   human ... IRAK2(3656)

Description

General description

The gene IRAK2 (interleukin 1 receptor associated kinase 2) encodes a member of the IRAK/Pelle family. It is an important signaling molecule of the TLR (Toll like receptor)/IL1-R (interleukin 1 receptor) superfamily. The members of IRAK family are characterized by the presence of an N-terminal DD (death domain) and a C-terminal Ser/Thr kinase or kinase-like domain. The gene is mapped to human chromosome 3p25.3-3p24.1.

Immunogen

synthetic peptide corresponding to amino acids 546-564 of human IL-1 receptor-associated kinase 2 (IRAK2).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The members of this family activate the transcription factor NF-κB (nuclear factor κ B) via IL-R1 (interleukin receptor type 1) and TLR (Toll-Like Receptor) mediated inflammatory/immune response. The receptor IL-R1 binds to its ligand proinflammatory cytokine IL-1, and this binding facilitates the association of IL-R1 with IL-1 receptor accessory protein (IL-1RAcP). This complex then recruits myeloid differentiation protein (MyD88), an intracellular TIR-containing adaptor. MyD88, then recruits IRAK1, IRAK2, IRAK4 and IRAK-M. These kinases interact with TRAF6 (TNF receptor-associated factor 6), which in turn links to NFκB-inducing kinase (NIK). NIK activates the IκB kinase complex (IKKα and IKKβ), which in turn phosphorylates IκB. This final phosphorylation leads to ubiquitin-proteasome-mediated degradation resulting in the activation of NF-κB.

Safety & Documentation

Safety Information

WGK Germany 
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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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I1529 Anti-IRAK2, C-Terminal antibody produced in rabbit, ~0.5 mg/mL, IgG fraction of antiserum

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