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I17002 Sigma-Aldrich

Interleukin-2 human

IL-2, recombinant, expressed in HEK 293 cells, suitable for cell culture, endotoxin tested

Synonym: Interleukin-2 human, Interleukin-2, T-cell growth factor

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Properties

Related Categories Cell Culture, Growth Factors and Cytokines, Interleukin-2, Interleukins (IL), Reagents and Supplements More...
Quality Level   200
recombinant   expressed in HEK 293 cells
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
potency   ≤4 ng/mL ED50
mol wt   15 kDa (glycosylated)
application(s)   cell culture | mammalian: suitable
suitability   endotoxin tested
storage temp.   −20°C

Description

General description

Recombinant human IL-2 is expressed in human 293 cells as a glycoprotein with a calculated moleculaer mass of 15 kDa. This protein is manufactured in human cells using an all-human production system, with full chemically defined ingredients and with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports better stability of the protein in culture.

Biochem/physiol Actions

Interleukin-2 (IL-2) is a lymphokine that promotes the long-term proliferation of activated T cells, and as such it is an essential mediator of immune response. IL-2 signals through the IL-2 receptor, a complex consisting of three chains, termed alpha, beta and gamma. The gamma chain is shared by all members of this family of cytokine receptors. Enhancement of IL-2 signaling using IL-2/anti-IL-2 immune complexes substantially improves immune surveillance in the context of suppressed immunity, and enhances control of the infection.

Interleukin-2 (IL-2) is an immunomodulatory factor produced by certain subsets of T lymphocytes. This lympho­kine promotes long term growth of acti­vated T cells and related cell types. IL-2 plays a role in the activation and proliferation of NK cells, induces γ-inter­feron and B cell growth factor secretion, and modulates the expression of the IL-2 receptor.

Sequence

APTSSSTKKTQLQLEHLLLDLQMILNGINNYKNPKLTRMLTFKFYMPKKATELKHLQCLEEELKPLEEVLNLAQSKNFHLRPRDLISNINVIVLELKGSETTFMCEYADETATIVEFLNRWITFCQSIISTLT

Physical form

Supplied as a lyophilized powder containing phosphate buffered saline.

Analysis Note

The biological activity of recombinant human IL-2 was tested in culture by measuring its ability to stimulate proliferation of the mouse CTLL-2 cells (mouse cytotoxic T cell line).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

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Protocols & Articles

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