• USA Home
  • I2018 - Monoclonal Anti-Insulin antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
I2018 Sigma-Aldrich

Monoclonal Anti-Insulin antibody produced in mouse

clone K36AC10, ascites fluid

Synonym: Insulin Antibody - Monoclonal Anti-Insulin antibody produced in mouse, Insulin Antibody

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies for Pancreatic Stem Cells,
conjugate   unconjugated
clone   K36AC10, monoclonal
biological source   mouse
application(s)   dot blot: suitable
  immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,000 using human or animal pancreas
  radioimmunoassay: suitable
species reactivity   monkey, pig, canine, rabbit, rat, feline, guinea pig, bovine, horse, human, sheep
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG1
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   P01308
Gene Information   human ... INS(3630)

Description

General description

The antibody reacts specifically against insulin by RIA and immunocytochemistry. It exhibits cross-reactivity with human proinsulin. The antibody binds to insulin with an affinity constant of 8.8 × 109 M-1 in RIA.

The insulin gene is mapped to human chromosome 11p15.5.

Application

Monoclonal Anti-Insulin antibody produced in mouse has been used in immunoblotting assay and ELISA.

Monoclonal Anti-Insulin antibody produced in mouse was used in ELISA-based protein pin array assay to study the interactions between human αB crystalline and insulin. It was used for flow cytometric analysis of rat white blood cells and mast cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways. Abnormal insulin secretion or function leads to diabetes mellitus, obesity and other carbohydrate metabolism disorders. Insulin is known to stimulate cell division.

Insulin is a major regulatory hormone important in the metabolic processes, transport of glucose, amino acids and certain ions. It is also involved in the synthesis of nucleic acids and proteins. The biological effects of insulin are mediated by insulin receptor and it integrates multiple major signaling pathways.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?