• USA Home
  • I2033 - Anti-phospho-Insulin Receptor/Insulin-Like Growth Factor-1 Receptor (pTyr1158/1162/1163) antibody produced in rabbit

EMAIL THIS PAGE TO A FRIEND
I2033 Sigma-Aldrich

Anti-phospho-Insulin Receptor/Insulin-Like Growth Factor-1 Receptor (pTyr1158/1162/1163) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-IR/IGF1R

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Hormones,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.1-1.0 mg/mL using stimulated CHO-T cells
species reactivity   human
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P08069
Gene Information   human ... IGF1R(3480)

Description

General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. IGF 1R is fully activated by phosphorylation of tyrosine residues at Tyr1158, Tyr1162 and Tyr1163. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1R signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-phospho-IR/IGF1R (pTyr1158/1162/1163) specifically recognizes human receptor and insulin-like growth factor-1 receptor phosphorylated at tyrosines 1158, 1162 and 1163. It may cross react with mouse and rat IR/IGF1R (100% homology).

Immunogen

synthetic phosphopeptide derived from the region of IR that contains tyrosines 1158, 1162, and 1163.

Application

Anti-phospho-IR/IGF1R (pTyr1158/1162/1163) antibody may be used for immunoblotting at a working concentration of 0.1-1.0 μg/ml using whole cell lysates from CHO-T cells.

Physical form

Solution in Dulbecco′s phosphate buffered saline, pH 7.3, containing 50% glycerol, 1 mg/mL BSA (IgG and protease-free) and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?