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I2143 Sigma

Anti-Interleukin-6 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-IL-6

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Description

Immunogen

Purified human recombinant IL-6 produced in E. coli.

General description

Interleukin-6 (IL-6) is a cytokine produced by activated T cells, endothelial cells, fibroblasts and activated monocytes or macrophages. It plays a crucial role in regulating the acute phase response as well as responses occurs after injury and infection. It interacts with IL-2 and facilitates in the proliferation of T lymphocytes. Anti-Interleukin-6 antibody can be used for selective neutralization of human IL-6 bioactivity in cell culture. It can also be used in radioimmunoassay (RIA) (diluted 1:60,000). Rabbit anti-Interleukin-6 antibody reacts specifically with natural and recombinant human IL-6 but not with recombinant human IL-1β, recombinant human TNF-α or recombinant mouse IL-6.

Physical form

Solution, 0.2 μm filtered, in 0.5 mL phosphate buffered saline, pH 7.4.

Specificity

The antibody will neutralize the biological activity of human IL-6 in cell culture. The antibody does not cross-react with human IL-1β, TNF-α or mouse IL-6.

Application

Anti-Interleukin-6 antibody can be used in western blotting and ELISA for studying human IL-6. It can also be used for dot blot immunoassay.

Features and Benefits

Antibody Bioguarantee
Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Peer-Reviewed Papers
15

References

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