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I2143 Sigma-Aldrich

Anti-Interleukin-6 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-IL-6

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Description

General description

Anti-Interleukin 6 (IL-6) is produced in rabbit using purified human recombinant IL-6 produced in E. coli as the immunogen. Interleukin-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. Interleukin-6 (IL-6) is a cytokine produced by activated T cells, endothelial cells, fibroblasts and activated monocytes or macrophages.

Specificity

The antibody will neutralize the biological activity of human IL-6 in cell culture. The antibody does not cross-react with human IL-1β, TNF-α or mouse IL-6.

Immunogen

Purified human recombinant IL-6 produced in E. coli.

Application

Anti-Interleukin-6 antibody can be used in western blotting and ELISA for studying human IL-6. It can also be used for dot blot immunoassay. Anti-Interleukin-6 antibody can be used for selective neutralization of human IL-6 bioactivity in cell culture. It can also be used in radioimmunoassay (RIA) (diluted 1:60,000).

It may also be used in immunoblotting.

Physical form

Solution, 0.2 μm filtered, in 0.5 mL phosphate buffered saline, pH 7.4.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Interleukin 6 (IL-6) acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. Interleukin 6 (IL-6) also potentiates the proliferative effect of Interleukin 3 (IL-3) on multipotential hematopoietic progenitors.

Interleukin-6 (IL-6) is a cytokine produced by activated T cells, endothelial cells, fibroblasts and activated monocytes or macrophages. It plays a crucial role in regulating the acute phase response as well as responses occurs after injury and infection. It interacts with IL-2 and facilitates in the proliferation of T lymphocytes.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

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Peer-Reviewed Papers
15

References

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