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  • I2158 - Anti-phospho-Insulin Receptor/Insulin-Like Growth Factor-1 Receptor (pTyr1162/1163) antibody produced in rabbit

I2158 Sigma-Aldrich

Anti-phospho-Insulin Receptor/Insulin-Like Growth Factor-1 Receptor (pTyr1162/1163) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Hormones,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: 1:1000 using CHO T-cells transfected with a vector containing insulin receptor and stimulated with insulin.
species reactivity   human, rat
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P08069
Gene Information   human ... IGF1R(3480)
rat ... Igf1r(25718)


General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. IGF 1R is fully activated by phosphorylation of tyrosine residues at Tyr1162 and Tyr1163. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1R signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-phospho-IR/IGF1R (pTyr1162/1163) specifically recognizes human and rat insulin receptor and insulin-like growth factor-1 receptor phosphorylated at tyrosines 1162 and 1163.


synthetic phosphopeptide derived from the region of IR that contains tyrosines 1162 and 1163.


Anti-phospho-IR/IGF1R (pTyr1162/1163) antibody may be used for immunoblotting at a working antibody dilution of 1:1000. The antibody is suitable for immunocytochemistry.

Target description

The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif corresponding to Tyr1158, 1162, and 1163 (for IR) and Tyr1131, 1135, and 1136 (for IGF1R). Autophosphorylation within the activation loop proceeds in a processive manner.

Physical form

Anti-phospho-IR/IGF1R (pTyr1162/1163) is supplied as a solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier and 0.05% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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