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I2643 Sigma-Aldrich

Insulin human

recombinant, expressed in yeast (proprietary host)

Purchase

Description

Application

Insulin human has been used:
• in the culture medium needed for the adipogenesis of ASCs (adipose-derived stem cells)
• to study the effect of insulin in the young and aged brains of rats.
• to study the effect of insulin on the inflammatory actions of protease-activated receptor 2 (PAR2) using mice model.

Packaging

25, 50, 250 mg in poly bottle

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
RTECS 
NM8900250

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What solvents can be used to make a solution of Insulin human, Product I2643?
Insulin is readily soluble in dilute acid and alkalies.The solubility of insulin is tested in 0.01 M hydrochloric acid at a concentration of 20 mg/mL.
How can I store the solutions of Insulin human, Product I2643,and how long?
A stock solution can be stored frozen at -20°C in single-use aliquots. Freeze-thaw cycles should be avoided.Alternatively, it can be stored for up to 12 months at 2-8°C if it is sterile filtered through a low protein binding membrane or if it contains a suitable bacteriostat, such as 0.1% thimerosal or sodium azide.
How many units of insulin are present in my vial of Insulin human, Product I2643?
The potency is listed by lot on the Certificate of Analysis, which can be accessed through our Advanced Search Options.Generally, product I2643 has a potency of 27.5 units/mg.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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