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I2786 Sigma-Aldrich

Interleukin-6 human

IL-6, recombinant, expressed in E. coli, Carrier Free

Synonym: B cell stimulating factor (BSF-2), IL-6, IL6, Interleukin-6 human, hepatocyte stimulating factor (HSF), interferon b2 (IFNb2), interleukin hybridoma/plasmacytoma-1 (IL-HP1), monocyte derived human B cell growth factor, plasmacytoma growth factor (PCT-GF)

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Properties

Related Categories CNTF Family - includes ciliary neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin-6 (IL-6), Cell Culture, Growth Factors and Cytokines, Interleukin-6, Interleukins (IL),
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   >97% (SDS-PAGE)
form   lyophilized powder
potency   0.2-2.0 ng/mL Biological Activity EC50
quality   Carrier Free
mol wt   26 kDa
packaging   pkg of 10 μg
storage condition   avoid repeated freeze/thaw cycles
impurities   ≤1 EU/μg protein Endotoxin Level
color   white
UniProt accession no.   P05231
storage temp.   −20°C
Gene Information   human ... IL6(3569)

Description

General description

Interleukin-6 (IL-6) is a multi-functional 26 kDa protein originally discovered in the medium of RNA-stimulated fibroblastoid cells1. IL-6 appears to be directly
involved in the immune responses that occur after infection and cellular injury, and it may prove to be as important as IL-1 and TNF-a in regulating the acute phase response2,3.

IL-6 is reportedly produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells including fibroblasts, myeloid progenitor cells, T cells, B cells, and hepatocytes. IL-6 induces multiple effects as indicated by its numerous synonyms4. In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes5. IL-6 potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors6.This product is lyophilized from a 0.2 mm-filtered solution of phosphate buffered saline, pH 7.4, containing a stabilizer.

The product was prepared from raw materials verified to be carrier free.

Application

Human interleukin-6 (IL-6) has been used to study the effects of IL-6, along with IL-8 and IL-1β, on the clot structure of healthy blood.

Biochem/physiol Actions

IL-6 is a multifunctional protein originally discovered in the media of cells stimulated with double stranded RNA. IL-6 appears to be directly involved in the responses that occur after infection and injury and may prove to be as important as IL-1 and TNF-α in regulating the acute phase response. IL-6 is reported to be produced by fibroblasts, activated T cells, activated monocytes or macrophages, and endothelial cells. It acts upon a variety of cells, including fibroblasts, myeloid progenitor cells, T cells, B cells and hepatocytes. IL-6 induces multiple effects, as indicated by its numerous synonyms: plasmacytoma growth factor (PCT-GF), interferon-β-2 (IFN-β2), monocyte derived human B cell growth factor, B cell stimulating factor (BSF-2), hepatocyte stimulating factor (HSF), Interleukin Hybridoma/Plasmacytoma-1 (IL-HP1). In addition, IL-6 appears to interact with IL-2 in the proliferation of T lymphocytes. IL-6 also potentiates the proliferative effect of IL-3 on multipotential hematopoietic progenitors.

Citation

References
1. Kishimoto, T., and Hirano, T, A new interleukin with pleiotropic activities. Bioessays, 9, 11-15 (1988).
2. Gauldie, J. et al., Interferon beta 2/B-cell stimulatory factor type 2 shares identity with monocyte-derived hepatocyte-stimulating factor and regulates the major acute phase protein response in liver cells. Proc. Natl. Acad. Sci. USA, 84, 7521-7255 (1987).
3. Van Snick, J., Interleukin-6: an overview. Ann. Rev. Immunol., 8, 253-278 (1990).
4. Kishimoto, T., IL-6: from laboratory to bedside. Clin.Rev. Allergy Immunol., 28, 177-186 (2005).
5. Nordan, R. et al., Purification and NH2-terminal sequence of a plasmacytoma growth factor derived from the murine macrophage cell line P388D1. J.Immunol., 139, 813-817 (1987).
6. Van Snick, J. et al., Purification and NH2-terminal amino acid sequence of a T-cell-derived lymphokine with growth factor activity for B-cell hybridomas. Proc. Natl. Acad. Sci. USA, 83, 9679-9683 (1986).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Protocols & Articles

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Peer-Reviewed Papers
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