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I3536 Sigma-Aldrich

Insulin human

recombinant, expressed in yeast, γ-irradiated, suitable for cell culture

Synonym: Insulin human

  • CAS Number 11061-68-0

  • Empirical Formula (Hill Notation) C257H383N65O77S6

  • Molecular Weight 5807.57

  •  MDL number MFCD00131380

  •  NACRES NA.77

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Properties

Quality Level   200
recombinant   expressed in yeast
sterility   γ-irradiated
form   lyophilized powder
potency   ≥25 USP units per mg
application(s)   cell culture | mammalian: suitable
solubility   0.01 M HCl: 20 mg/mL, clear, colorless to faintly yellow
shipped in   ambient
storage temp.   −20°C
InChI key   PBGKTOXHQIOBKM-FHFVDXKLSA-N
Gene Information   human ... INS(3630)

Description

General description

The INS gene encodes for preproinsulin, which is enzymatically converted into insulin. Insulin is produced in the insulin-producing pancreatic β cells. Preproinsulin is converted to proinsulin in ER and proinsulin is then proteolytically processed to form insulin in newly-forming insulin secretory granules. Insulin production is tightly regulated by specific DNA elements present within ∼400 bp in the proximal region of the INS promoter.

Application

Insulin human has been used for the following applications:
• For adipogenic differentiation assays (in the preparation of medium supplement)
• Incubation of cells for the evaluation of the effects of insulin
• Mass spectrometry (used for the external caliberation)

Biochem/physiol Actions

Insulin is responsible for two types of actions- excitatory and inhibitory. In its excitatory role, it increases the uptake of glucose and lipid synthesis, and in its inhibitory role it inhibits glycogenolysis, gluconeogenesis, lipolysis, proteolysis and ketogenesis. Aberrant insulin secretion leads to various disorders such as diabetes, hyperglycemia or hypoglycaemia. Type I diabetes is a result of autoimmune destruction of β cells of pancreas, which leads to depletion of insulin. Mutant INS-gene Induced Diabetes of Youth (MIDY) syndrome is an autosomal dominant disorder caused by missense mutations, which lead to aberrant proinsulin folding. Impaired glucose tolerance (IGT) or non-insulin-dependent diabetes mellitus (NIDDM) is caused by resistance to insulin-stimulated glucose uptake.

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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