I4269 Sigma-Aldrich

Interleukin-4 human

IL-4, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

Synonym: IL-4, hIL-4



Related Categories Cancer Research, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Core Bioreagents,
Quality Level   200
biological source   human
recombinant   expressed in E. coli
assay   ≥98% (SDS-PAGE)
form   lyophilized powder
potency   ≤0.400 ng/mL EC50
quality   endotoxin tested
mol wt   14.9 kDa (containing 129 amino acid residues)
packaging   pkg of 5 and 50 μg
application(s)   cell culture | mammalian: suitable
impurities   ≤1.000 EU/μg
color   white
UniProt accession no.   P05112
storage temp.   −20°C
Gene Information   human ... IL4(3565)
mouse ... IL4(3565)


General description

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including differentiation of naive T cells into the TH2 phenotype, promoting B cell proliferation, antibody isotype switching, and expression of other TH2 cytokines including IL-5 and IL-9.1,2 IL-4 plays a critical role in the development of allergic inflammation and asthma.3

IL-4 binds to two distinct receptors; the type I receptor, a heterodimer consisting of the IL-4Rα chain and the common gamma chain, γc, and the type II receptor, a heterodimer of IL-4Rα and IL-13Rα1.4

Naturally occurring IL-4 has a molecular mass of 12-20 kDa. Recombinant human IL-4 is a 14.9 kDa protein containing 129 amino acid residues. Human and mouse IL-4 share a 50% amino acid sequence homology, but their biological actions are species-specific.5


Lymphokine with profound effects on the growth and differentiation of immunologically competent cells, inhibits VEGF-induced and βFGF-induced angiogenesis.

Physical form

Lyophilized from a sterile filtered solution.

Analysis Note

The biological activity is measured by the dose-dependent stimulation of the proliferation of human TF-1 cells.


1. Paul, W.E., Interleukin-4: a prototypic immuno-regulatory lymphokine. Blood, 77, 1859-1870 (1991).
2. O′Garra, A., and Arai, N., The molecular basis of T helper 1 and T helper 2 cell differentiation. Trends Cell Biol., 10, 542-550 (2000).
3. Ryan, J.J. et al., Mast cell homeostasis: a fundamental aspect of allergic disease. Crit. Rev. Immunol., 27, 15-32 (2007).
4. Callard, R.E. et al., IL-4 and IL-13 receptors: are they one and the same? Immunol. Today, 17, 108-110 (1996).
5. Yokota, T. et al., Molecular biology of interleukin 4 and interleukin 5 genes and biology of their products that stimulate B cells, T cells and hemopoietic cells. Immunol. Rev., 102, 137-187 (1988).
6. Kitamura, T. et al., Establishment and characterization of a unique human cell line that proliferates dependently on GM-CSF, IL-3, or erythropoietin. J. Cell Physiol., 140, 323-334 (1989).

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Single- & multi-channel pipettes from BRAND

Pipette Controller from Brand
Protocols & Articles


Interleukin Family (IL)

The Interleukins comprise a disparate group of cytokines and growth factors that are produced by and released from leukocytes. Interleukin-1β (1L-1β) is released primarily from stimulated macrophages...
Jennifer Fries
BioFiles 2009, 4.5, 16.
Keywords: Anti-inflammatory agents, Antitumor agents, Cancer, Cell proliferation, Gene expression, Growth factors, Transcription

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?