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I4401 Sigma-Aldrich

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, cell culture tested

Synonym: IFN-αA/D

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Properties

Related Categories Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥95%
form   buffered aqueous solution
mol wt   19 kDa
packaging   pkg of 100K units
impurities   endotoxin, tested
color   colorless
suitability   cell culture tested
UniProt accession no.   P01563
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... IFNA2(3440)

Description

Application

Interferon-αA/D human has been used:
• for identification of coronavirus replicase inhibitors
• in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
• in combination with vitamin D to check reduction in HCV protein production in cell culture

Biochem/physiol Actions

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physical form

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide
Protocols & Articles

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Peer-Reviewed Papers
15

References

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