• USA Home
  • I4401 - Interferon-αA/D human

I4401 Sigma-Aldrich

Interferon-αA/D human

≥95%, recombinant, expressed in E. coli, buffered aqueous solution, cell culture tested

Synonym: IFN-αA/D



Related Categories Bioactives/Supplements, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Cytokines and Growth Factors,
biological source   human
recombinant   expressed in E. coli
assay   ≥95%
form   buffered aqueous solution
mol wt   19 kDa
packaging   pkg of 100K units
impurities   endotoxin, tested
color   colorless
suitability   cell culture tested
UniProt accession no.   P01563
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... IFNA2(3440)



Interferon-αA/D human has been used:
• for identification of coronavirus replicase inhibitors
• in combination with ACH-806 [1-(4-pentyloxy-3-trifluoromethylphenyl)-3-(pyridine-3-carbonyl)thiourea] to study the effect on hepatitis C virus (HCV) replication
• in combination with vitamin D to check reduction in HCV protein production in cell culture

Biochem/physiol Actions

Interferon-αA/D (Bg/II), also known as Universal Type I Interferon, is an α interferon hybrid that crosses the species barrier. The Bg/II denotes the restriction enzyme site from which this hybrid is constructed from recombinant human interferons αA and αD. Interferon-αA/D has been proven to be active on many mammalian cells. It can substitute for human, monkey, mouse, bovine, rat, cat, dog, rabbit, sheep, goat, horse, pig or hamster type I interferon (α, β, ω and τ). Type I Interferons are a closely related family of 165-172 amino acid proteins that are produced by leukocytes (α subtypes), fibroblasts (β subtypes), lymphocytes (ω subtypes), and ruminant embryos (τ subtypes). This cytokine has anti-viral, anti-proliferative, immunoregulatory, and proinflammatory activities.

Physical form

Solution in phosphate buffered saline containing 0.1% bovine serum albumin

Analysis Note

The biological activity is determined in the cytopathic effect inhibition assay using MDBK cells with vesicular stomatitis virus (VSV). A cytopathic effect of 50% is produced with 1 unit/ml interferon. The units are determined with respect to NIH international standard reference for human interferonαA.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis


Certificate of Origin


Request a Serum Sample

Cell Culture Guide
Protocols & Articles

Related Content

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?