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I4532 Sigma-Aldrich

Monoclonal Anti-Interleukin-13 antibody produced in rat

clone 38213.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-13

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
conjugate   unconjugated
clone   38213.11, monoclonal
biological source   rat
application(s)   capture ELISA: suitable
  neutralization: suitable
  western blot: 1-2 μg/mL
species reactivity   mouse
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
UniProt accession no.   P20109
Gene Information   mouse ... Il13(16163)

Description

General description

Interleukin-13 (IL-13) is a cytokine produced by Th2 cells and mediates the production of immunoglobulins, B cell proliferation and differentiation of cells of the monocytic lineage. The interaction of IL-13 with its receptor triggers responses via the STAT-6 pathway and results in antibody-mediated responses, eosinophil recruitment in lungs, airway remodelling, mucus overproduction, secretion of matrix metalloproteinases (MMP)-9 and MMP-12 and IgE production. The other signaling axis that is mediated by IL-13 is the EGFR-TGF-α pathway that induces proliferation of epithelial cells. Elevated IL-13 has been associated with the symptoms of asthma and is detected in elevated levels in the airways and sputum of asthma patients
Monoclonal Anti-Mouse Interleukin-13 recognizes recombinant mouse IL-13.

Specificity

The biological activity is measured by its ability to neutralize recombinant mouse IL-13.

Immunogen

purified recombinant mouse IL-13 expressed in E. coli.

Application

Monoclonal anti-mouse interleukin-13 may be used in neutralization assays at a working concentration range of 03.-0.8 μg/ml. For immunoblotting a concentration of 1-2 μg/ml may be used. For ELISA a working concentration of 2-4 μg/ml is recommended.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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