• USA Home
  • I4753 - Invertase from Candida utilis

EMAIL THIS PAGE TO A FRIEND
I4753 Sigma-Aldrich

Invertase from Candida utilis

Grade X, ≥300 units/mg solid

Synonym: β-D-Fructofuranoside fructohydrolase, β-Fructofuranosidase, Saccharase

Purchase

Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Application Index, Biochemicals and Reagents, Carbohydrate Hydrolysis,
type   Grade X
mol wt   ~260 kDa
foreign activity   α-galactosidase (melibiase), essentially free
storage temp.   −20°C

Description

General description

Invertase from Candida utilis is secreted in the periplasm. The molecular weight of invertase from C. utilis is 300 kDa. It is a dimer of identical subunits. It is a N-glycosylated protein, rich in mannose oligosaccharide in its structure. The invertase encoding gene of C. utilis shares high homology with SUC2 gene of Saccharomyces cerevisiae.

Application

Invertase from Candida utilis has been used:
• as a supplement in YE liquid medium to observe for morphological changes in Streptomyces sp.
• to determine the sucrose content in ethanolic extract of leaf tissues
• to determine the total non-structural carbohydrates (TNC) in root tissues

This enzyme is useful for enzymatic determination of saccharose and for the structure investigation of carbohydrates containing β-D-fructofuranoside residue.

Packaging

1 g in poly bottle

Biochem/physiol Actions

Invertase acts on sucrose and cleaves it into glucose and fructose. It is used in the preparation of invert syrup with equimolar concentration of fructose and sucrose. It finds its applications in confectionaries and in the production of alcoholic beverages, glycerol and lactic acid. Invertase is also used in the preparation of artificial honey, plasticizers for cosmetic industries.

Physical properties

Michaelis constant : 1.5x10 -2M (Saccharose)
Structure : Glycoprotein containing ca. 50% of carbohydrates
Optimum pH : 3.5 - 4.0 Optimum temperature : 60 - 70oC
pH Stability : pH 4.0 - 6.0 (50oC, 10min)
Thermal stability : below 60oC (pH 4.5, 10min)
Substrate specificity : The enzyme hydrolyzes saccharose and raffinose, but does not
hydrolyze inulin and melezitose.6)

Unit Definition

One unit will hydrolyze 1.0 μmole of sucrose to invert sugar per min at pH 4.5 at 55°C.

Preparation Note

Chromatographically purified

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Please Enter a Lot Number

Certificate of Origin

Please Enter a Lot Number
Protocols & Articles

Protocols

Assay Procedure for Invertase

The appearance of reducing sugars is measured by the modified Fehling-Lehmann-Schoorl method.7)
Keywords: Indicators, Titrations

Enzymatic Assay of Invertase

This procedure applies to all products that have specification for the enzymatic activity of Invertase at Sigma-Aldrich Saint Louis.
Keywords: Biochemistry, Enzyme activity, Enzymology

Related Content

Enzymes & Proteins

Application Index | Enzyme Index | Substrate Index | Inhibitor Index | Cofactor Index | Lectin Index
Keywords: Cell signaling, Diagnostic, Drug discovery, Metabolomics, Molecular biology

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?