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  • I5032 - Anti-IKKγ/NEMO (C-Terminal) antibody produced in rabbit

I5032 Sigma-Aldrich

Anti-IKKγ/NEMO (C-Terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym: Anti-IKKAP1



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: 0.5-1 μg/mL
species reactivity   mouse, human, rat
mol wt   antigen ~52 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
UniProt accession no.   Q9Y6K9
Gene Information   human ... IKBKG(8517)
mouse ... Ikbkg(16151)
rat ... Ikbkg(309295)


General description

The gene IKKγ, also referred to as IKBKG (inhibitor of κ light polypeptide gene enhancer in B-cells, kinase γ) encodes the regulatory subunit of the inhibitor of κB kinase (IKK) complex, which also contains two catalytic subunits-IKK1/IKKα and IKK2/IKKβ. By phosphorylating IκB proteins, the IKK complex mediates the activation of NF (nuclear factor)-κB in response to a variety of stimuli. The gene is mapped to human chromosome Xq28.


synthetic peptide corresponding to amino acids 400-416 of human IKKγ.

Physical form

Solution in phosphate buffered saline containing 0.02% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The gene IKKγ, also referred to as NEMO (NF-κB essential modulator) interacts with IKKβ and is required for the activation of the IKK complex. Upon stimulation, IKK phosphorylates, ubiquitinates and degrades the NF-κB inhibitors, IκBα and IκBβ. This releases NF-κB, which in turn, enters the nucleus and activates target genes involved in immune and inflammatory responses. Mutations in this gene have been associated with incontinentia pigmenti, anhidrotic ectodermal dysplasia with immunodeficiency (EDA-ID), and other immunodeficiencies.

Features and Benefits

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Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis


Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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