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I5513 Sigma-Aldrich

Imidazole

for molecular biology, ≥99% (titration)

Synonym: 1,3-Diaza-2,4-cyclopentadiene, Glyoxaline

  • CAS Number 288-32-4

  • Empirical Formula (Hill Notation) C3H4N2

  • Molecular Weight 68.08

  •  Beilstein/REAXYS Number 103853

  •  EC Number 206-019-2

  •  MDL number MFCD00005183

  •  eCl@ss 39161001

  •  PubChem Substance ID 24896072

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Properties

Related Categories Biochemicals, Biochemicals and Reagents, Biological Buffers, Buffers / Buffer Salts, Buffers A to Z,
grade   for molecular biology
vapor pressure   <1 mmHg ( 20 °C)
InChI Key   RAXXELZNTBOGNW-UHFFFAOYSA-N
assay   ≥99% (titration)
form   crystalline powder
pH   6.2-7.8 (25 °C)
pKa (25 °C)   6.95
bp   256 °C(lit.)
mp   88-91 °C (lit.)
Featured Industry   Diagnostic Assay Manufacturing
foreign activity   DNase, RNase and protease, none detected

Description

General description

Imidazole is a heterocyclic compound with a five-membered planar ring. It is amphoteric and highly polar. The pharmacophore of imidazole exists in bioactive compounds including amino acids, plant growth regulators and therapeutic agents.

Application

Imidazole has been used
• in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein
• in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography
• as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells

Excellent for buffers in the range of pH 6.2-7.8

Packaging

5, 25, 100 g in glass bottle

Biochem/physiol Actions

Imidazole derivatives have antibacterial, antifungal and anticancer functionality. It interacts with DNA and also binds to protein and stops cell division. It also acts as a microtubule destabilizing agents and inhibits topoisomerase and Cytochrome P450 Family 26 Subfamily A Member 1 (CYP26A1) enzymes. Imidazole based anticancer drug find applications in cancer chemotherapy. It is used as buffer component for purification of the histidine tagged recombinant proteins in immobilized metal-affinity chromatography (IMAC).

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3263 8 / PGIII
WGK Germany 
2
RTECS 
NI3325000
Flash Point(F) 
293 °F
Flash Point(C) 
145 °C
Protocols & Articles

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Peer-Reviewed Papers
15

References

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