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I5523 Sigma-Aldrich

Insulin from porcine pancreas

powder, ≥27 USP units/mg

Synonym: Insulin from hog pancreas

  • CAS Number 12584-58-6

  • Empirical Formula (Hill Notation) C256H381N65O76S6

  • Molecular Weight 5777.54

  •  EC Number 235-703-3

  •  MDL number MFCD00131380

  •  NACRES NA.32

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Properties

Related Categories Biochemicals and Reagents, Cell Biology, Cell Signaling and Neuroscience, Cytokines, Growth Factors and Hormones, Hormones,
Quality Level   200
biological source   Porcine pancreas
form   powder
specific activity   ≥27 USP units/mg
application(s)   cell culture | mammalian: suitable
solubility   0.01 M HCl: 5 mg/mL, clear, colorless
cation traces   Zn: 0.20-0.70%
shipped in   ambient
storage temp.   −20°C
InChI key   NOESYZHRGYRDHS-ZYCCASTOSA-N
Gene Information   pig ... INS(397415)

Description

Application

Insulin from porcine pancreas has been used:
• for culturing of the primary tumors obtained from mice and porcine preantral follicles
• to characterize hormones involved in postnatal chicken preadipocyte differentiation in vitro
• to check the reduction ability of the Escherichia coli thiol disulfide exchange proteins (DsbA, DsbC, DsbG) towards insulin

Packaging

10, 50 mg in glass bottle

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Other Notes

InChI: 1S/C256H381N65O76S6/c1-29-131(23)205(313-193(337)104-258)251(391)317-204(130(21)22)247(387)288-159(75-82-200(347)348)217(357)282-156(71-78-189(262)333)221(361)308-183-116-401-402-117-184-242(382)305-178(111-323)239(379)294-162(88-123(7)8)224(364)295-168(95-140-53-61-146(327)62-54-140)227(367)283-154(69-76-187(260)331)218(358)290-161(87-122(5)6)222(362)285-158(74-81-199(345)346)220(360)302-174(101-190(263)334)234(374)298-170(97-142-57-65-148(329)66-58-142)230(370)309-182(241(381)304-176(255(396)397)103-192(265)336)115-400-399-114-181(213(353)272-107-194(338)278-153(72-79-197(341)342)216(356)281-151(51-42-84-270-256(266)267)211(351)271-108-195(339)279-166(93-138-46-36-32-37-47-138)226(366)297-167(94-139-48-38-33-39-49-139)229(369)299-171(98-143-59-67-149(330)68-60-143)237(377)320-208(136(28)326)253(393)321-85-43-52-186(321)245(385)286-152(50-40-41-83-257)214(354)277-134(26)254(394)395)311-249(389)203(129(19)20)316-235(375)164(90-125(11)12)292-228(368)169(96-141-55-63-147(328)64-56-141)296-223(363)160(86-121(3)4)289-209(349)133(25)276-215(355)157(73-80-198(343)344)287-246(386)202(128(17)18)315-236(376)165(91-126(13)14)293-232(372)173(100-145-106-269-120-275-145)301-238(378)177(110-322)280-196(340)109-273-212(352)180(113-398-403-118-185(310-243(183)383)244(384)319-207(135(27)325)252(392)306-179(112-324)240(380)318-206(132(24)30-2)250(390)312-184)307-225(365)163(89-124(9)10)291-231(371)172(99-144-105-268-119-274-144)300-219(359)155(70-77-188(261)332)284-233(373)175(102-191(264)335)303-248(388)201(127(15)16)314-210(350)150(259)92-137-44-34-31-35-45-137/h31-39,44-49,53-68,105-106,119-136,150-186,201-208,322-330H,29-30,40-43,50-52,69-104,107-118,257-259H2,1-28H3,(H2,260,331)(H2,261,332)(H2,262,333)(H2,263,334)(H2,264,335)(H2,265,336)(H,268,274)(H,269,275)(H,271,351)(H,272,353)(H,273,352)(H,276,355)(H,277,354)(H,278,338)(H,279,339)(H,280,340)(H,281,356)(H,282,357)(H,283,367)(H,284,373)(H,285,362)(H,286,385)(H,287,386)(H,288,387)(H,289,349)(H,290,358)(H,291,371)(H,292,368)(H,293,372)(H,294,379)(H,295,364)(H,296,363)(H,297,366)(H,298,374)(H,299,369)(H,300,359)(H,301,378)(H,302,360)(H,303,388)(H,304,381)(H,305,382)(H,306,392)(H,307,365)(H,308,361)(H,309,370)(H,310,383)(H,311,389)(H,312,390)(H,313,337)(H,314,350)(H,315,376)(H,316,375)(H,317,391)(H,318,380)(H,319,384)(H,320,377)(H,341,342)(H,343,344)(H,345,346)(H,347,348)(H,394,395)(H,396,397)(H4,266,267,270)/t131-,132-,133-,134-,135+,136+,150-,151-,152-,153-,154-,155-,156-,157-,158-,159-,160-,161-,162-,163-,164-,165-,166-,167-,168-,169-,170-,171-,172-,173-,174-,175-,176-,177-,178-,179-,180-,181-,182-,183-,184-,185-,186-,201-,202-,203-,204-,205-,206-,207-,208-/m0/s1

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
RTECS 
NM8895000
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Guidelines for Bioassays on MultiScreen Filter Plates: Protein Binding and Cell Loading

The MultiScreen Assay System is well suited for a variety of separation techniques, including cell separations and solid phase bioassays. The MultiScreen system makes it possible to carry out an enti...
Keywords: Buffers, Cell culture, Cell proliferation, Chromatography, Filtration, Individual protein Immunoprecipitation, Precipitation, Protein assay, Reductions, Sample preparations, Scintillation, Separation

Plasma Derived Proteins and Enzymes

Despite their complexity, blood and plasma are abundant biological resources for the discovery of drug targets and biomarkers for human disease. It is estimated that plasma may contain as many as 40,...
BioFiles 2006, 1.5, 2.
Keywords: Applications, Gas chromatography, Type

Reversed-Phase HPLC Analysis of Insulin Variants and Analogs by UV and Mass Spectral Detection

Insulin is an approximately 6000 Da peptide hormone used to regulate the glucose concentration in blood. There are several different forms of insulin that are both natural and recombinant; the recomb...
Cory Muraco, Stacy Shollenberger, and Hillel Brandes
MilliporeSigma, Bellefonte, PA USA
Keywords: Chromatography, High performance liquid chromatography, Hormones, Mass spectrometry, Pharmaceutical, Phase transitions, Reversed-phase chromatography, Separation

Peer-Reviewed Papers
15

References

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