• USA Home
  • I5527 - Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse

I5527 Sigma

Monoclonal Anti-Interleukin-1 Soluble Receptor Type I antibody produced in mouse

clone 35730, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-1 sRI




recombinant human IL-1 sRI expressed in Sf 21 cells.

General description

Interleukin 1 (IL-1) is a cytokine responsible for the regulation of inflammation and immune responses. Interleukin-1 Receptor Type I is a 80kD protein found on many cells like monocytes, NK cells, T cells, B cells, fibroblasts, dendritic cells, endothelial cells, vascular endothelial cells, neural cells, basophils, neutrophils and eosinophils. It has a pivotal role in signal transduction, where IL-1 Receptor Type I binds with interleukin-1 and the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex that facilitates IL-1 dependent activation of NF-κ-B, MAPK and other pathways. Monoclonal anti-Interleukin-1 Soluble Receptor Type I antibody can be used in western blotting. Mouse anti-Interleukin-1 Soluble Receptor Type I antibody reacts specifically with human IL-1 Receptor I. The product has shown no cross-reactivity with recombinant human IL-1 ra, recombinant human IL-1 RII, recombinant human IL-1 RacP (IL-1 R3), recombinant human IL-1 R5, recombinant human IL- 1 R6 or recombinant mouse IL-1 RI.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.


Monoclonal anti-Interleukin-1 Soluble Receptor Type I antibody can be used as a capture antibody in a sandwich ELISA.

Price and Availability

Antibody Explorer

Explore with Confidence

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?