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  • I5652 - Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

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I5652 Sigma-Aldrich

Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

clone 22722, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-2 sRα

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
conjugate   unconjugated
clone   22722, monoclonal
biological source   mouse
application(s)   immunocytochemistry: 8-25 μg/mL using using human PBMCs
  immunohistochemistry (frozen sections): 8-25 μg/mL using using human tissue
  neutralization: suitable
  western blot: 1 μg/mL (under non-reducing conditions)
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
UniProt accession no.   P01589
Gene Information   human ... IL2RA(3559)

Description

Immunogen

recombinant human IL-2 soluble receptor alpha (IL-2 Rα), expressed in Sf 21 insect cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline wth 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. The effects of IL-2 are mediated by the signaling activated by its binding to IL-2 receptor (IL-2 R). IL-2 activates key signaling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle.

Preparation Note

Purified using protein A.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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