• USA Home
  • I5652 - Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

I5652 Sigma

Monoclonal Anti-Interleukin-2 Soluble Receptor α antibody produced in mouse

clone 22722, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-2 sRα



Related Categories Alphabetical Index, Antibodies, Antibodies against Cytokines, Chemokines, and Growth Factors for Stem Cell Biology, Antibodies against Cytokines/Growth Factors/Chemokines for Stem Cell Biology, Antibodies for Cell Biology,
species reactivity   human
application(s)   immunocytochemistry: 8-25 μg/mL using using human PBMCs
  immunohistochemistry (frozen sections): 8-25 μg/mL using using human tissue
  neutralization: suitable
  western blot: 1 μg/mL (under non-reducing conditions)
clone   22722, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1
storage temp.   −20°C
Gene Information   human ... IL2RA(3559)
biological source   mouse
conjugate   unconjugated



recombinant human IL-2 soluble receptor alpha (IL-2 Rα), expressed in Sf 21 insect cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline wth 5% trehalose.

Preparation Note

Purified using protein A.

Biochem/physiol Actions

Interleukin-2 (IL-2) is a cytokine produced by activated T cells in response to antigen stimulation. The effects of IL-2 are mediated by the signaling activated by its binding to IL-2 receptor (IL-2 R). IL-2 activates key signaling pathways such as MAPK, JAK/STAT as well as PI3K/Akt. Activation of these pathways modulates production of inflammatory cytokines, survival and progression through cell cycle.

Price and Availability

Antibody Explorer

Explore with Confidence

Milli-Q® Water Purification Solutions
Safety & Documentation


Certificate of Analysis

Certificate of Origin

Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?