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  • I6152 - Anti-Interleukin-2 Soluble Receptor α antibody produced in goat

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I6152 Sigma-Aldrich

Anti-Interleukin-2 Soluble Receptor α antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-IL-2 sRα

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Description

Specificity

The antibody will neutralize human cell surface IL-2 receptor α mediated-bioactivity. The antibody shows ≤15% cross-reactivity with recombinant human IL-2 Rγ, ≤ 5% cross-reactivity with recombinant human IL-6 sR, and ≤1% cross-reactivity with recombinant human IL-1 sRII and IL-2 sRβ.

Immunogen

recombinant human IL-2 soluble receptor α, expressed in Sf 21 insect cells.

Application

Anti-Interleukin-2 Soluble Receptor α antibody may be used in immunoblotting at a working concentration of 0.1-0.2 μg/ml. For flow cytometry, the recommended concentration is 2-3 μg/106 cells. The antibody is suitable for IL-2 sR α neutralization assays.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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