• USA Home
  • I6385 - Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
I6385 Sigma-Aldrich

Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse

clone MB-11, ascites fluid

Synonym: Anti Human IgM Antibody - Monoclonal Anti-Human IgM (μ-chain specific) antibody produced in mouse, Anti Human Igm Antibody, Monoclonal Anti-Human IgM (μ-chain specific)-Alkaline Phosphatase

  •  NACRES NA.46

Purchase

Properties

Related Categories Alphabetical Index, Antibodies, HU-HZ, Human IgM Secondary Antibodies and Conjugates, Primary Antibodies,
conjugate   unconjugated
clone   MB-11, monoclonal
biological source   mouse
application(s)   indirect ELISA: 1:5,000
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgG2b
Quality Level   300
antibody product type   secondary antibodies
contains   15 mM sodium azide

Description

General description

Human serum-derived IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents.
Immunoglobulin M (IgM) antibodies appear early in the course of infections. IgM antibodies are responsible for agglutination of red blood cells in mis-matched blood transfusions. The level of IgM may vary with the status of disease or infection.

IgM is a highly conserved antibody in vertebrates and is expressed early during immune response. It exists as a pentamer and is secreted by peritoneal B cells.

Mouse monoclonal clone MB-11 anti-Human IgM (μ-chain specific) antibody is specific for the μ-chain of human IgM as determined by an ELISA. It does not bind human IgG, IgA, or light chains.

Application

Mouse monoclonal clone MB-11 anti-Human IgM (μ-chain specific) antibody may be used to identify and quantitate the level of human IgM in vitro and in human serum or other biological fluids by immunochemical or immunohistological techniques. Elisa assays were performed using alkaline phosphatase conjugated anti-human IgM to detect IgMs in phage supernatants and human sera at 1:1000 dilution.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?