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  • I7153 - Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit

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I7153 Sigma-Aldrich

Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies for Pancreatic Stem Cells,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunoprecipitation (IP): 4 μg using 0.5 mg of a 3T3/A31 RIPA lysate
  western blot: 0.5-2 μg/mL using RIPA lysates of 3T3/A31 mouse fibroblasts
species reactivity   rat, human, mouse
mol wt   antigen 165 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   P35568
Gene Information   human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)

Description

General description

Insulin receptor substrate 1 (IRS1) gene is mapped to human chromosome 2q36.3. The gene encodes a 185 kDa protein and is ubiquitously expressed. Irs1 belongs to the group of cytoplasmic adaptor proteins.

Immunogen

C-terminal 14 amino acid residues of rat liver insulin receptor substrate 1.

Application

Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in immunoprecipitation using 0.5 mg of a 3T3/A31 RIPA lysate.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl, and 0.05% sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Insulin receptor substrate 1 (IRS-1) is phosphorylated on multiple tyrosine residues. It plays a crucial role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways. Over expression of IRS-1can leads to mammary tumorigenesis and metastasis. Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in western blotting using RIPA lysates of 3T3/A31 mouse fibroblasts. Rabbit anti-Insulin Receptor Substrate 1 (IRS-1) antibody reacts specifically with insulin receptor substrate 1 (165 kD). The product has shown species cross reactivity with rat, mouse and human IRS-1.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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