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  • I7153 - Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit

I7153 Sigma

Anti-Insulin Receptor Substrate 1 (IRS-1) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies for Pancreatic Stem Cells,
species reactivity   human, mouse, rat
application(s)   immunoprecipitation (IP): 4 μg using 0.5 mg of a 3T3/A31 RIPA lysate
  western blot: 0.5-2 μg/mL using RIPA lysates of 3T3/A31 mouse fibroblasts
clone   polyclonal
antibody form   affinity isolated antibody
form   buffered aqueous solution
mol wt   antigen mol wt 165 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... IRS1(3667)
mouse ... Irs1(16367)
rat ... Irs1(25467)
biological source   rabbit
conjugate   unconjugated



C-terminal 14 amino acid residues of rat liver insulin receptor substrate 1.

General description

Insulin receptor substrate 1 (IRS-1) is a 160kD adapter protein which binds to activated insulin receptor and is phosphorylated on multiple tyrosine residues. It plays a crucial role in transmitting signals from the insulin and insulin-like growth factor-1 (IGF-1) receptors to intracellular pathways. Over expression of IRS-1can leads to mammary tumorigenesis and metastasis. Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in western blotting using RIPA lysates of 3T3/A31 mouse fibroblasts. Rabbit anti-Insulin Receptor Substrate 1 (IRS-1) antibody reacts specifically with insulin receptor substrate 1 (165 kD). The product has shown species cross reactivity with rat, mouse and human IRS-1.

Physical form

Solution in 0.1 M Tris-glycine, pH 7.4, containing 0.15 M NaCl, and 0.05% sodium azide.


Anti-Insulin Receptor Substrate 1 (IRS-1) antibody can be used in immunoprecipitation using 0.5 mg of a 3T3/A31 RIPA lysate.

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RIDADR  NONH for all modes of transport
WGK Germany  nwg
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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