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I7651 Sigma-Aldrich

Monoclonal Anti-Interleukin-4 antibody produced in mouse

clone 34019.111, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-4

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies to Cytokines and Growth Factors, Antibodies to Interleukins (IL),
conjugate   unconjugated
clone   34019.111, monoclonal
biological source   mouse
application(s)   indirect ELISA: suitable
  neutralization: suitable
  western blot: suitable
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2b
antibody product type   primary antibodies
UniProt accession no.   P05112
Gene Information   human ... IL4(3565)

Description

General description

Interleukin-4 also refers as T cell growth factor-2 (TCGF-2), mast cell growth factor-2 (MCGF-2) or B cell stimulatory factor-1 (BSF-1) is a 12-20 kD complex glycoprotein produced by a subset of activated T cells. It has a pivotal role in the growth and differentiation of immunologically competent cells. Monoclonal anti-Interleukin-4 antibody can be used for neutralizing the biological activity of human IL-4 (rhIL-4). Mouse anti-Interleukin-4 antibody shows no cross-reactivity with recombinant mouse IL-4.

Specificity

The antibody will neutralize the biological activity of recombinant human IL-4. The antibody shows no cross-reactivity with recombinant mouse IL-4.

Immunogen

recombinant human IL-4.

Application

Monoclonal anti-Interleukin-4 antibody can be used in indirect ELISA and western blotting.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Purified using protein A.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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