• USA Home
  • I7901 - Monoclonal Anti-Interleukin-6 antibody produced in mouse

EMAIL THIS PAGE TO A FRIEND
I7901 Sigma

Monoclonal Anti-Interleukin-6 antibody produced in mouse

clone 6708.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-IL-6

Purchase

Description

Immunogen

recombinant human IL-6.

General description

Interleukin-6 is a pleotropic cytokine involved in hematopoiesis, inflammation and immune response. IL-6 is secreted by macrophages, dendritic cells and B cells, in response to TNF-α, PGDF and IL-1. The signaling by IL-6 is mediated by heterodimeric receptor, IL-6R, comprising of IL-6Rα (soluble factor) and gp130 (sometimes called IL-6Rβ). gp130 is crucial component for downstream signal transduction. In response to IL-6, gp130 activates kinases of JAK family, STATs, MAPK and Ras proteins. IL-6-mediated signaling is essential for normal development of T and B cells and has been implicated in many diseases such as rheumatoid arthritis, Crohn disease, sepsis, fever, systemic lupus erythematosus, osteoporosis and insulin resistance. Monoclonal Anti-Interleukin-6 antibody recognizes human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.

Physical form

Lyophilized from 0.2 μm filtered solution in phosphate buffered saline.

Specificity

The antibody will neutralize the biological activity of recombinant human IL-6. The antibody shows no cross-reactivity with recombinant mouse IL-6 or recombinant human IL-6 sR, OSM, LIF CNTF, or IL-11 or recombinant horse gp130.

Preparation Note

Purified using protein A.

Application

Monoclonal Anti-Interleukin-6 antibody may be used for ELISA at a minimum antibody concentration of 4 μg/ml. For immunoblotting a working antibody concentration of 1-2 μg/ml may be used. The antibody may be used in neutralization assays; ND50 is 0.01-0.15 μg/ml. For neutralization of IL-6 from human peripheral blood mononuclear cells, a concentration of 2.5 μg/ml was used.

Price and Availability


Find Interleukin 6 Antibodies
Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
RIDADR  NONH for all modes of transport
WGK Germany  3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary & Secondary Antibodies

Search & filter by validated application (ie, WB, IF/ICC, IHC, ELISA, etc,) host species, clonality (monoclonal, polyclonal and recombinant) and more. Learn from technical information, product highli...
Keywords: Amplification, Buffers, Enzyme-linked immunosorbent assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers
15

References

Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?