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I8028 Sigma

Anti-IRAK antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym: Anti-Interleukin-1 Receptor-Associated Kinase



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation, Antibodies for Gene Regulation and Expression,
species reactivity   human
application(s)   immunoprecipitation (IP): 2-4 μg using 0.5 mg of human HeLa or THP-1 cell extracts
  western blot: 0.25 μg/mL using human HeLa or THP-1 cell extracts
clone   polyclonal
antibody form   IgG fraction of antiserum
form   buffered aqueous solution
mol wt   antigen mol wt 80 kDa (in non-activated cells)
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... IRAK1(3654)
biological source   rabbit
conjugate   unconjugated



synthetic peptide corresponding to amino acids 700-712 of the human IL-1 receptor-associated kinase (IRAK).

General description

The gene IRAK1 (interleukin 1 receptor associated kinase 1) encodes a member of the IRAK family. The members of this family consist of a conserved N-terminal death domain and a central kinase domain. The gene is mapped to human chromosome Xq28.

Physical form

Solution in phosphate buffered saline, containing 0.02% sodium azide.

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WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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