I8260 Sigma

IgM from human serum

reagent grade, ~95% (HPLC), buffered aqueous solution

Synonym: Human IgM



Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
grade   reagent grade
assay   ~95% (HPLC)
form   buffered aqueous solution
conjugate   unconjugated
shipped in   wet ice
storage temp.   2-8°C



1, 5 mg in glass bottle

10 mg in serum bottle

Physical form

Solution in 0.05 M Tris-HCl, 0.2 M sodium chloride, pH 8.0, containing 15 mM sodium azide.


Purified human IgM may be used as a reference antigen, standard, blocking agent, or coating protein in a variety of immunoassays including ELISA, dot immunobinding, Western immunoblotting, immunodiffusion, and immunoelectrophoresis. Other applications include starting materials for the preparation of immunogens and solid phase immunoadsorbents. IgM from human serum was used as standard in ELISA (100 μg/ml) and dot blot assay. Antibody concentration range of 200 μg/ml to 1 mg/ml was used in IgM binding assays.

General description

IgM antibodies are present as pentamers in the serum and are produced in response to antigens
IgM from human serum is purified from normal human serum by precipitation and gel filtration techniques. The immunoglobulin is determined to be atleast 95% pure by HPLC procedures.

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Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

UHPLC Analysis of Human IgM on BIOshell™ IgG 1000 Ã… C4

From our library of Articles, Sigma-Aldrich presents UHPLC Analysis of Human IgM on BIOshell™ IgG 1000 Ã… C4

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