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  • I8773 - Anti-Insulin-Like Growth Factor-I antibody produced in goat

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I8773 Sigma-Aldrich

Anti-Insulin-Like Growth Factor-I antibody produced in goat

affinity isolated antibody

Synonym: Anti-IGF-I

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Cytokines and Growth Factors,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   neutralization: 3-12 μg/mL
  western blot: 0.1-0.2 μg/mL
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   100
antibody product type   primary antibodies
UniProt accession no.   P05019
Gene Information   human ... IGF1(3479)

Description

General description

Insulin-like growth factors -1 and -2 are important regulators of signalling that mediates growth and metabolism in cells. Insulin-like growth factor -1 shares structural homology with proinsulin and is mainly produced by liver. IGF-1 acts via specific receptor, IGF-1R, a heterodimeric tyrosine kinase receptor that signals to various second messenger pathways such as MAPK, Raf/Ras and PI3K cascades. The effect of IGF-1 is by endocrine, paracrine and autocrine signalling. The effects of IGF-1 signalling are important at many developmental levels from cells to organ to organism, growth, differentiation, anti-apoptosis, proliferation and transformation. Irregular IGF-1 signaling has been implicated in many cancers including melanoma, breast, lung, colon and prostate
Anti-IGF-I recognizes human insulin-like growth factor I. The antibody does not react with human IGF-II.

Specificity

The antibody neutralizes the biological activity of recombinant human IGF-I.

Immunogen

recombinant human IGF-I expressed in Escherichia coli

Application

Anti- IGF-1 antibody may be used for neutralization reactions, the ND50 of the antibody is 3-12 μg/ml. For immunoblotting a working antibody concentration of 0.1-0.2 μg/ml.

Physical form

Lyophilized from a 0.2 μm filtered solution of phosphate buffered saline, pH 7.4, with 5% trehalose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Preparation Note

Affinity isolated by IGF-I affinity chromatography.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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