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  • I9031 - Monoclonal Anti-Intracellular Adhesion Molecule 3 antibody produced in mouse

I9031 Sigma-Aldrich

Monoclonal Anti-Intracellular Adhesion Molecule 3 antibody produced in mouse

~1 mg/mL, clone 186-2G9, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-ICAM-3



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells, Antibodies for Stem Cell Biology,
conjugate   unconjugated
clone   186-2G9, monoclonal
biological source   mouse
application(s)   direct immunofluorescence: 2 μg using 1 mg protein lysate
  flow cytometry: 2 μg using 1 mg protein lysate
  immunohistochemistry: 2-4 μg/mL using human tonsil tissue sections
  immunoprecipitation (IP): 2 μg using 1 mg protein lysate
  western blot: 1-5 μg/mL
species reactivity   human
mol wt   antigen 120 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   purified immunoglobulin
Quality Level   200
antibody product type   primary antibodies
concentration   ~1 mg/mL
UniProt accession no.   P32942
Gene Information   human ... ICAM3(3385)


General description

The gene ICAM3 (intercellular adhesion molecule 3) encodes type I membrane glycoprotein that belongs to the immunoglobulin (ig) superfamily. The encoded protein is extensively glycosylated and contains five Ig-like domains that share high homology with corresponding domains in ICAM-1 and ICAM-2. ICAM3 is found to be expressed on all leukocytes, including the resting leukocytes. The gene is mapped to human chromosome 19p13.2.


stimulated human leukocytes.

Physical form

Solution in phosphate buffered saline pH 7.4, containing 0.08% sodium azide.

Biochem/physiol Actions

The protein intercellular adhesion molecule 3 (ICAM-3) functions as a receptor for LFA-1 (leukocyte function-associated antigen-1) and mediates adhesive interactions of leukocyte cell surface adhesion molecules in inflammatory and immune response. LFA-1 participates in several processes, such as T cell-mediated killing, T helper and B lymphocyte responses, natural killing, Ab-dependent cellular cytotoxicity by monocytes and granulocytes, and adherence of leukocytes to endothelial cells, fibroblasts, and epithelial cells. ICAM-3 mediates the adhesion of resting T lymphocytes to LFA-1 and participates in the initiation of immune responses. It may also function in lymphocyte activation, as well as in adhesion.

Safety & Documentation

Safety Information

NONH for all modes of transport


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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