I9140 Sigma-Aldrich

IgG from goat serum

technical grade, ≥80% (SDS-PAGE), buffered aqueous solution

Synonym: Goat IgG



Related Categories Antibodies, Normal Human and Animal Immunoglobulins, Purified Immunoglobulins, Sera and Immunoglobulins, Supplementary Products More...
grade   technical grade
assay   ≥80% (SDS-PAGE)
form   buffered aqueous solution
application(s)   ChIP: suitable
  immunohistochemistry: suitable
conjugate   unconjugated
shipped in   dry ice
storage temp.   −20°C



Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


10, 50, 100 mg in glass bottle

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.2 containing 15 mM sodium azide


In immunohistochemical applications, IgG from goat serum can be used as a blocking agent (500 μg/ml in paraformaldehyde-fixed mouse uterus sections) or as a negative control (1:56000 in pig cecal tissue).

Technical grade IgG may be used as an economical alternative to the reagent grade immunoglobulins, in applications where high purity is not required. Goat IgG was used as negative control antibody in immunohistochemical staining of cecal tissue of pigs at a dilution of 1:56000. Goat IgG was used in immunohistochemistry of rat brain sections at a concentration of 2 μg/ml. The antibody was also used in chromatin immunoprecipitation and as control in infection inhibition assay.

General description

Goat IgG is a glycoprotein antibody that is fractionated from pooled normal goat serum. In immunoelectrophoresis, goat IgG yields a single major arc of precipitation in the γ region against anti-goat IgG and anti-goat whole serum. Minor γ arcs may also be observed against anti-goat whole serum.

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections
Goat IgG is purified from normal goat serum by fractionation.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis

Certificate of Origin

Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary and Secondary Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay needs. Use the Antibody...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?