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  • I9156 - Monoclonal Anti-Itk antibody produced in mouse

I9156 Sigma

Monoclonal Anti-Itk antibody produced in mouse

~1 mg/mL, clone 2F12, purified immunoglobulin, buffered aqueous solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, IL2-Inducible T-Cell Kinase (Itk),
species reactivity   mouse, human
application(s)   immunoprecipitation (IP): 2 μg using 1 mg protein lysate
  western blot: 1 μg/mL
clone   2F12, monoclonal
concentration   ~1 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 72 kDa
shipped in   wet ice
storage temp.   −20°C
Gene Information   human ... ITK(3702)
mouse ... Itk(16428)
biological source   mouse
conjugate   unconjugated



recombinant protein encoding the N-terminus of murine Itk.

Physical form

Solution in phosphate buffered saline pH. 7.4, containing 0.08% sodium azide.

Price and Availability

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Safety & Documentation

Safety Information

Personal Protective Equipment  Eyeshields, Gloves
RIDADR  NONH for all modes of transport
WGK Germany  nwg
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Antibody Explorer | Buy Primary & Secondary Antibodies

Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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