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I9278 Sigma-Aldrich

Insulin solution human

Chemically defined, recombinant, expressed in Saccharomyces cerevisiae, sterile-filtered, BioXtra, suitable for cell culture

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Properties

Related Categories Apoptosis and Cell Cycle, Cell Biology, Cell Culture, Cell Signaling and Neuroscience, Hormones,
Quality Level   GMP
recombinant   expressed in Saccharomyces cerevisiae
sterility   sterile-filtered
product line   BioXtra
form   solution
suitability   suitable for cell culture
storage temp.   2-8°C

Description

Biochem/physiol Actions

Two-chain polypeptide hormone produced by the β-cells of pancreatic islets. Its molecular weight is ~5800 Da. The α and β chains are joined by two interchain disulfide bonds. The α chain contains an intrachain disulfide bond. Insulin regulates the cellular uptake, utilization, and storage of glucose, amino acids, and fatty acids and inhibits the breakdown of glycogen, protein, and fat.

Physical form

stable 2-8 °C

Application

Insulin solution human is recommended for use in cell culture applications at 0.5 to 1mL per liter of medium.
Insulin solution human has been used for insulin tolerance test in mice and rats. It has also been used for adipogenic differentiation of human embryonic stem cells (hESCs)-derived outgrowth cultures and hMSCs (human mesenchymal stromal cells).

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What is the concentration ofProduct I9278,Insulin solution human?
The acceptable concentration range of this insulin solution can be found on the specification sheet.The lot-specific result is reported on the Certificate of Analysis.
What is the molarity of Product I9278, Insulin solution human?
A 10mg/mL solution of human insulin has a molarity of 1.7 μmol/mL or 1.7 mM, using the molecular weight of 5807.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Cell Culture Guide
Protocols & Articles

Articles

Insulin Signaling and Energy Homeostasis

Glucose metabolism is regulated by the opposing actions of insulin and glucagon. Insulin is released from pancreatic ß cells in response to high blood glucose levels and regulates glucose metabolism ...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 2011
Keywords: Apoptosis, Biological processes, Carboxylations, Catalog, Diabetes, Gene expression, Gluconeogenesis, Glycolysis, Hormones, Metabolism, Metabolites, Transcription, Transduction, Type

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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