I9516 Sigma-Aldrich


BioReagent, for molecular biology, ≥99.5%

Synonym: sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

  • CAS Number 67-63-0

  • Linear Formula (CH3)2CHOH

  • Molecular Weight 60.10

  •  Beilstein Registry Number 635639

  •  EC Number 200-661-7

  •  MDL number MFCD00011674

  •  PubChem Substance ID 24896158



Related Categories 2-Propanol (Isopropanol), Core Bioreagents, DNA & RNA Purification, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis,
grade   for molecular biology
vapor density   2.1 (vs air)
vapor pressure   33 mmHg ( 20 °C)
  44 mmHg ( 25 °C)
product line   BioReagent
assay   ≥99.5%
form   liquid
autoignition temp.   750 °F
expl. lim.   2 %
impurities   ≤0.2% water
color   colorless
refractive index   n20/D 1.377(lit.)
bp   82 °C(lit.)
mp   −89.5 °C (lit.)
solubility   water: soluble (completely)
density   0.785 g/mL at 25 °C(lit.)
suitability   suitable for precipitation of nucleic acids
storage temp.   room temp


General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly.


25, 500 mL in glass bottle


2-Propanol has been used:
• in DNA and RNA extraction
• for determination of hydroxyproline levels
• for checking cell viability by MTT assay
• in microarray hybridization

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
UN1219 - class 3 - PG 2 - Isopropanol
WGK Germany 
Flash Point(F) 
53.6 °F
Flash Point(C) 
12 °C

Milli-Q® Water Purification Solutions
Protocols & Articles


Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Isopropanol Molecular Biology Reagent

CAS RN 67-63-0 EC 200-661-7 Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol
Keywords: Acylations, Biochemistry, Crystallization, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Molecular biology, Precipitation, Size-exclusion chromatography, Solvents

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...


Analyzing real-time PCR data by the comparative CT method

Two different methods of presenting quantitative gene expression exist: absolute and relative quantification. Absolute quantification calculates the copy number of the gene usually by relating the PC...
Keywords: Amplification, Anti-inflammatory agents, Apoptosis, Cancer, Clinical, Dehydration reaction, Diagnostic, Gene expression, Hormones, Methylations, Microarray Analysis, Molecular biology, Polymerase chain reaction, Polymerase chain reaction - quantitative, Reductions, Transcription, Transduction, Transfection, cDNA microarrays, transformation

MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

TRI Reagent® Protocol

1A. Tissue: Homogenize tissue samples in TRI Reagent (1 ml per 50–100 mg of tissue) in a Polytron® or other appropriate homogenizer.
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

The single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction: twenty-something years on

Since its introduction, the 'single-step' method has become widely used for isolating total RNA from biological samples of different sources. The principle at the basis of the method is that RNA is s...
Keywords: AGE, Absorption, Biochemistry, Cancer, Cell disruption, Centrifugation, Chromatography, Cloning, Degradations, Electrophoresis, Eliminations, Gel electrophoresis, Gene expression, Genetics, Homogenization, Molecular biology, Northern blot, Polymerase chain reaction, Precipitation, Separation

Related Content

Pressure-Temperature Nomograph Interactive Tool

Use the interactive controls above to simplify calculations and improve the efficiency of your distillation or evaporation requirements.
Keywords: Evaporation, Vaporization

Properties of Solvents Table

The table below presents the physical characteristics of common solvents. Easily reference or compare solvent properties like boiling point, density, and refractive index all from one list.
Keywords: Gas chromatography, Green chemistry, High performance liquid chromatography, Melting, Solvents

Peer-Reviewed Papers


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