I9516 Sigma-Aldrich


BioReagent, for molecular biology, ≥99.5%

Synonym: sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

  • CAS Number 67-63-0

  • Linear Formula (CH3)2CHOH

  • Molecular Weight 60.10

  •  Beilstein/REAXYS Number 635639

  •  EC Number 200-661-7

  •  MDL number MFCD00011674

  •  PubChem Substance ID 24896158

  •  NACRES NA.52



Related Categories 2-Propanol (Isopropanol), Core Bioreagents, DNA & RNA Purification, Life Science Reagents for DNA/RNA Electrophoresis, Life Science Reagents for Protein Electrophoresis,
Quality Level   200
grade   for molecular biology
vapor density   2.1 (vs air)
vapor pressure   33 mmHg ( 20 °C)
  44 mmHg ( 25 °C)
product line   BioReagent
assay   ≥99.5%
form   liquid
autoignition temp.   750 °F
expl. lim.   2.0-12.7 %, 93 °C
application(s)   DNA extraction: suitable
  RNA extraction: suitable
impurities   ≤0.2% water
color   colorless
refractive index   n20/D 1.377 (lit.)
bp   82 °C (lit.)
mp   −89.5 °C (lit.)
solubility   water: soluble (completely)
density   0.785 g/mL at 25 °C (lit.)
suitability   suitable for precipitation of nucleic acids
storage temp.   room temp
SMILES string   CC(C)O
InChI   1S/C3H8O/c1-3(2)4/h3-4H,1-2H3


General description

Isopropanol is a clear, colorless, polar organic solvent commonly used in chemistry and molecular biology laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. This product (2-propanol) is designated as molecular biology grade and is suitable for the precipitation of nucleic acids.

Protocols for the use of isopropanol:

• DNA precipitation from mammalian tissue, ethidium bromide extraction from DNA samples, and RNA isolation from cells and tissues.
• Protein crystallization.
• Isolate retinoids from biological matrices for HPLC and HPLC-MS analysis.


2-Propanol has been used:
• in DNA and RNA extraction
• for determination of hydroxyproline levels(The Merck Index, 12th ed)
• for checking cell viability by MTT assay
• in microarray hybridization
Suitable for use in DNA precipitation using standard protocols. Also suitable as a solvent for making solutions for molecular biology applications.


25, 500 mL in glass bottle

Safety & Documentation

Safety Information

Signal word 
Hazard statements 
Target organs 
Central nervous system
UN1219 - class 3 - PG 2 - Isopropanol
WGK Germany 
Flash Point(F) 
53.6 °F - closed cup
Flash Point(C) 
12.0 °C - closed cup
Protocols & Articles


Introduction to PAGE

Polyacrylamide gels are formed by the reaction of acrylamide and bis-acrylamide (N,N’-methylenebisacrylamide) that results in highly cross-linked gel matrix. The gel acts as a sieve through which the...
Keywords: Buffers, Cell disruption, Electrophoresis, Gel electrophoresis, PAGE, Protein electrophoresis, Sample preparations, Separation, Western blot

Isopropanol Molecular Biology Reagent

CAS RN 67-63-0 EC 200-661-7 Synonyms: 2-Propanol, sec-Propyl alcohol, IPA, Isopropyl alcohol
Keywords: Acylations, Biochemistry, Crystallization, High performance liquid chromatography, Liquid chromatography mass spectrometry, Mass spectrometry, Molecular biology, Precipitation, Size-exclusion chromatography, Solvents

Pressure-Temperature Calculator for Solvents

*Calculations provide estimated results based on Antoine's Coefficients and their associated temperature range values. Antoine's Coefficients were obtained from the National Institute of Standards an...


MystiCq® MicroRNA® Quantitation System

Technology Overview Workflow Background Protocols miRNA isolation cDNA synthesis miRNA qPCR Advanced information Product selection guides Troubleshooting
Keywords: AGE, Amplification, Buffers, Cell disruption, Centrifugation, Degradations, Diagnostic, Digestions, Diseases, Electrophoresis, Gel electrophoresis, Gene expression, Homogenization, Microarray Analysis, Molecular biology, Nucleic acid annealing, Nucleic acid denaturation, PAGE, Polymerase chain reaction, Polymerase chain reaction - quantitative, Precipitation, Purification, RNA purification, Sample preparations, Separation, Solvents, Titrations

Protocol for Anti Ago-RNA Immunoprecipitation from mammalian cells using the RIP kit

1.    Grow cells to ~80% confluency.  You will need ~2 million cells/RIP, or ~4 million cells for IgG (neg control) & Ago2 RIP
Keywords: Cell disruption, Immunoprecipitation, Individual protein Immunoprecipitation, RNA immunoprecipitation

Selecting Correctly Expressing Recombinants

Blue / White colony screening is a strategy to quickly and easily distinguish between recombinant and non-recombinant colonies. It requires a special vector and a special strain of E. coli. It is par...
Keywords: AGE, Amplification, Antibiotics, Cell disruption, Centrifugation, Cloning, Degradations, Detergents, Diagnostic, Digestions, Electrophoresis, Gel electrophoresis, Molecular biology, Peptide synthesis, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sequencing, Transfection, transformation

TRI Reagent® Protocol

TRI Reagent® solution (also sold as TRIzol) is a mixture of a mixture of guanidine thiocyanate and phenol in a monophase solution that is used for the isolation of DNA, RNA and protein from biologica...
Keywords: AGE, Buffers, Cell disruption, Centrifugation, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Homogenization, PAGE, Polymerase chain reaction, Precipitation, Sample preparations, Separation, Solvents, Western blot

Peer-Reviewed Papers


Related Products

related product

Product #


Add to Cart

D8418 Dimethyl sulfoxide, for molecular biology
B7906 1-Butanol, for molecular biology, ≥99%
P4557 Phenol solution, BioReagent, Equilibrated with 10 mM Tris HCl, pH 8.0, 1 mM EDTA, for molecular biology

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?